郏县红牛IGF2基因多态性与生长性状关系研究

本研究利用PCR-SSCP技术首次对郏县红牛母牛IGF2基因多态性及其与生长性状关系进行了研究。结果表明:IGF2基因第2外显子表现多态性,存在AA、AB、BB三种不同基因型。其频率分别为0.235/0.480/0.285;A/B等位基因频率分别为0.474/0.526;该位点纯合度为0.501,杂合度为0.499,位点间有效等位基因数为1.996。该基因位点处于Hardy-Weinberg平衡状态(P<0.05)。通过对郏县红牛IGF2基因第2外显子位点不同基因型与样本体重及其它体尺指标的关联分析表明:不同基因型对胸围(P<0.01)和体重(P<0.05)有显著影响,AB基因型个体的胸围和体重明显大于AA和BB型个体。说明IGF2基因可以作为标记用于郏县红牛的肉用选育。

Association of Polymorphism of IGF2 Gene with Growth Traits in Jiaxian Red Cattle

In this study,the polymorphisms of IGF2 gene were firstly detected by PCR-SSCP method and their association with growth traits was analyzed in Jiaxian Red cattle.Results showed that IGF2 gene demonstrated polymorphisms with three genotypes AA,AB and BB.The genotype frequencies were 0.235,0.480 and 0.285.Frequencies of alleles A and B were 0.474 and 0.526 respectively.The Ho,He and Ne of the locus were 0.501,0.499 and 1.996 respectively.The polymorphie locus of IGF2 gene was at Hardy-Weinberg equilibrium(P>0.05).The polymorphic locus was associated with the growth traits of Jiaxian cattle.There was significant effect of this polymorphism on body weight(P<0.05)and heart girth(P<0.01).The heart girth and body weight of the individuals with genotype AB were significantly higher than that with genotype AA and BB.This indicated that IGF2 gene as marker can be used to selec- tion of meat trait in Jiaxian Red cattle.