川东南马尾松外生菌根苗及其抗铝性研究

采自西南地区分离的马尾松林常见的土著菌株松乳菇(Lactarius deliciosus 01,03)、彩色豆马勃(Pisolithus tinctorius 715)和模式种双色蜡蘑(Laccaria bicolor S238N)的基质纯培养物及液体纯培养物,分别按混合(Mix=Ld 01 Ld 03 Pt 715 Lb S238N)及单独接种的方式接种1月龄马尾松(Pinus massoniana)幼苗.菌根苗在不同pH土壤及铝胁迫条件下培育3个月后采样分析其生长和养分吸收特性.结果表明:4种接种方式均能有效形成菌根,显著增加幼苗的生物量,促进幼苗对N、P、K的吸收并显著(P＜0.05)提高抗铝性;混合接种促进马尾松幼苗生长的效果较单独接种好,并且pH=6.1时,幼苗对K的吸收量最大,铝吸收量最少;pH=4.1时,幼苗对N、P的吸收效果最好,但对K的吸收和对铝的抗性最差.

Research on EMC Seedlings of Pinus massoniana and AI Resistance in Southeast of Sichuan

The matrix pure cultures and liquid pure culture of three common indigenous strains isolated from pinus massoniana of southwestern were Lactarius deliciosus(01,03),Pisolithus tinctorius 715 and the Laccaria bicolor S238N.With them,one-month seedlings of Pinus massoniana were inoculated by means of Co-inoculation(Mix=Ld 01 Ld 03 Pt 715 Lb S238N)and single inoculation respectively.The seedlings were then cultured under different pH conditions(pH4.1,pH6.1,pH6.7) and aluminum stress.Growth and the specific nutrient absorption of the seedlings were analyzed after three months.The results showed that Mycorrhizaee were formed effectively in all the tests,and the absorption of N,P,K were enhanced too,leading to an significant biomass increase and aluminum resistance for the seedlings(P<0.05).Co-inoculation was more effective on accelerating the growth of the seedlings compared with single inoculation.When pH6.1 the seedling showed best absorption of K and least of Al,while there were best absorption of N,P,least of K,and worst aluminum resistance when pH4.1.