曼地亚红豆杉中紫杉醇等与产地因子的相关性

从国内8个不同产地采集曼地亚红豆杉Taxus media枝条和林下土壤，利用高效液相色谱（HPLC）法测定了紫杉醇（taxol）和10-脱乙酰基巴卡亭Ⅲ（10-deacetylbaccatin，10-DAB）质量分数，用常规方法测定了土壤的矿质元素及肥力结构等。结果表明：不同产地的曼地亚红豆杉紫杉醇和10-DAB质量分数差异很大，紫杉醇有随北纬纬度增高而递减的趋势；紫杉醇和10-DAB与多数土壤因子相关性不显著，其中：紫杉醇与有机质呈显著正相关，与北纬纬度呈显著负相关，10-DAB与土壤矿质元素钴和锰质量分数呈显著负相关；紫杉醇与10-DAB之间不存在相关性（r = - 0.197）；用多元逐步回归分析建立了紫杉醇质量分数的回归方程。推测：曼地亚红豆杉的紫杉醇及其衍生物质量分数变化规律与红豆杉属其他种相似，受生长必需环境因子影响明显。图2表3参23

Study on the correlation between taxol,10-DAB of Taxus media and its major soil factors

To know the difference of taxol,10-deacetylbaccatin Ⅲ（10-DAB） content of Taxus media at different site,about 500 g more branch and leaf of 10 individuals,2 500 g more soil each sampled from eight different domestic origins-Qiuchuang,Jingcheng,Dongwu,Renhe,Shanghu in Zhejiang,Tianpen in Sichuang,Luolong in Henan,Xiaotangshan in Beijing-were collected,the taxol and 10-DAB content were measured by high-performance liquid chromatography（HPLC）,and soil mineral elements content and soil structure were determined by standard methods.Then,with the data,a stepwise multiple regression analysis by stepwise and a partial correlation analyses by the temperature when sampling as control variable were conducted.Results showed that taxol content decreased along the north latitude（north latitude from 28°55′27″ up to 40°11′39″,taxol content from 0.330 4 g·kg-1 down to 0.030 6 g·kg-1）.Also,taxol and 10-DAB were merely correlated with most soil factors;taxol was significantly（P0.031） and positively correlated（r = 0.800） with soil organic matter,but significantly（P0.047） and negatively correlated（r =-0.761） with site north latitude;10-DAB was significantly（P0.026） and negatively correlated with mineral elements Co（r = 0.813） and Mn（P0.030,r =-0.801）;there was no linear correlation between taxol and 10-DAB（r =-0.197）;and the regression equation established was：y = 0.067-0.003 x1 ＋ 0.000 012 08 x2-0.001 x3.Overall,taxol content,as a derivative of T.media,was similar to other Taxus species such as Taxus wallichiana var.mairei,with its content changing largely with soil organic matter.