7个猪种MyoD基因家族中3个基因外显子的SNPs检测分析

本研究利用PCR SSCP技术,以7个猪种为研究对象,根据GenBank上猪的MyoG、MyoD和Myf5基因序列设计了17对引物,对此三基因的外显子进行了SNPs检测。检测结果:MyoG和MyoD两个基因均未检测到多态;在Myf5基因的3个外显子上都检测到了多态:第1外显子上有1个点突变G→C;第2外显子上有3个点突变C→A、A→G、G→A;第3外显子上,3387bp处有1个碱基A缺失,3417bp处有3个碱基T缺失,3443bp处有1个点突变T→C。Myf5基因的高度多态性,说明7个猪种含有丰富的遗传变异信息,具有很高的选择潜力。

SNPs Detection of the Exons of Three Genes of MyoD Gene Families in Seven Pig Breeds

The study subject included 7 swine breeds. According to the sequences of MyoG, MyoD and Myf5 of swine in GenBank, seventeen pairs of primers for MyoG, MyoD and Myf5 were designed. PCR-SSCP technology was applied to detect SNPs of the exons of the three genes. The results showed that no polymorphism exists in MyoG and MyoD, and some SNPs in three exons of Myf5. There was one mutant site in the first exon of Myf5(G→C); There were three mutant sites in the second exon of Myf5 (C→A, A→G and G→A); in the third exon of Myf5, one base A deletion at 3387 bp, three bases T deletion at 3417 bp, one mutant site at 3443 bp(T→C).The high polymorphism of Myf5 showed that seven swine breeds were rich in genetic variation, and had high selective competency.