| 兔多杀性巴氏杆菌株外膜蛋白A基因克隆及序列分析 |
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| 引用本文： | 庞安娜,鲍国连,刘燕,韦强,肖琛闻,季权安.兔多杀性巴氏杆菌株外膜蛋白A基因克隆及序列分析[J].浙江农业学报,2010,22(6):741. |
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| 作者姓名： | 庞安娜 鲍国连 刘燕 韦强 肖琛闻 季权安 |
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| 作者单位： | 1.浙江师范大学 化学与生命科学学院,浙江 金华 321004；;2.浙江省农业科学院 畜牧兽医研究所,浙江 杭州 310021 |
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| 基金项目： | 国家现代兔产业技术体系项目，国家公益性行业(农业)科研专项项目 |
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| 摘 要： | 参考GenBank中登录的多杀性巴氏杆菌序列设计了一对特异性引物,采用PCR方法分别扩增出兔多杀性巴氏杆菌C51-2-499株、C51-3-500株的OmpA全长片段,克隆到pMD18-T载体上进行序列测定和分析。结果表明：OmpA全长1068 kb,含有一个1068 kb的开放阅读框(ORF),编码353个氨基酸,相对分子量为37 969.8 kD,等电点pI为8.90。与6株参考菌株比较,核苷酸同源性为897%～99.1%,氨基酸同源性为82.9%～98.6%。
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| 关 键 词： | 兔多杀性巴氏杆菌 外膜蛋白A 序列分析 |
Sequence analysis of outer membrane protein A gene of rabbit Pasteurella multocida |
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| PANG An-na,BAO Guo-lian,LIU Yan,WEI Qiang,XIAO Chen-Wen,JI Quan-an.Sequence analysis of outer membrane protein A gene of rabbit Pasteurella multocida[J].Acta Agriculturae Zhejiangensis,2010,22(6):741. |
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| Authors: | PANG An-na BAO Guo-lian LIU Yan WEI Qiang XIAO Chen-Wen JI Quan-an |
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| Institution: | 1College of Chemistry and Life Science, Zhejiang Normal University,Jinhua 321004, China；2Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China |
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| Abstract: | According to OmpA gene of Pasteurella multocida published in GenBank, a pair of primers was designed. Using the primers, full\|length OmpA of C51-2-499, C51-3-500 was amplified by PCR. It was cloned into pMD18-T vector and then sequenced. The result showed that the full-length of OmpA was 1.068 kb containing a 1.068 kb open reading frame. The relative molecular weight of the deduced amino acid sequence (223 amino acid residues) was 37 969.8 kD and the pI value was 8.90. The alignment results showed that the nucleotide sequence homologies were 89.7%-99.1% and the deduced amino acid homologies were 82.9%-98.6% compared with six reference OmpA sequences of Pasteurella multocida published in GenBank． |
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| Keywords: | rabbit Pasteurella multocida outer membrane protein A sequence analysis |
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