| 猪UCP3基因的分离与多态分析 |
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| 引用本文： | 朱宝芹,任亮,苏玉虹. 猪UCP3基因的分离与多态分析[J]. 畜牧与兽医, 2006, 38(6): 14-17 |
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| 作者姓名： | 朱宝芹 任亮 苏玉虹 |
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| 作者单位： | 锦州医学院辽宁省分子细胞生物学与新药开发重点实验室,辽宁,锦州,121000 |
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| 基金项目： | 辽宁省科技厅博士启动基金(20021072),辽宁省教育厅基金(2004F112) |
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| 摘 要： | 解偶联蛋白3是一种线粒体内膜上的转运蛋白,可以使氧化过程与ADP磷酸化过程解偶联。本研究分离了猪UCP3基因的外显子2~3和外显子6。经过序列分析,发现猪UCP3基因共有3处框移突变和18个SNP位点。在猪UCP3片段中分别包含一个SmaⅠ和SduⅠ酶切位点。UCP3片段SmaⅠ位点在大白×梅山F2家系中经PCR-RFLP分析,表现为AA、AB和BB 3种基因型,A基因频率为0.57,B基因频率为0.43。因此,可以将UCP3基因作为猪分子遗传育种的候选基因。
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| 关 键 词： | 猪 解偶联蛋白3 PCR-RFLP 遗传效应分析 |
| 文章编号： | 0529-5130（2006）06-0014-04 |
| 收稿时间： | 2005-08-10 |
| 修稿时间： | 2005-08-10 |
The isolation of uncoupling protein-3 gene of pigs and analysis of its polymorphism |
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| ZHU Bao-qin,REN Liang,SU Yu-hong. The isolation of uncoupling protein-3 gene of pigs and analysis of its polymorphism[J]. Animal Husbandry & Veterinary Medicine, 2006, 38(6): 14-17 |
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| Authors: | ZHU Bao-qin REN Liang SU Yu-hong |
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| Abstract: | Uncoupling protein 3(UCP3) was mitochondrial membrane transporters involved in themogenesis,which uncoupled the oxidation and phosphorylation of ADP on respiration chain.Exon 2～3 and 6 of UCP3 gene were isolated in this study.By DNA sequences analysis,three frame-shift mutation and eighteen SNP were found in UCP3 gene of pigs.There were restriction enzyme sites of SmaⅠand SduⅠ in UCP3 gene of pigs.The characterization of the SmaⅠ polymorphism in UCP3 gene was analyzed by PCR-RFLP among "Largewhite×Meishan" F2 group.The genotypes of UCP3 were AA、AB and BB.The frequencies of A allele is 0.57 and B is 0.43. |
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| Keywords: | pig UCP3 PCR-RFLP association analysis |
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