Plant regeneration in sweet potato (Ipomoea batatas L., Convolvulaceae) |
| |
Authors: | D Sihachakr R Haïcour JM Cavalcante Alves I Umboh D Nzoghé A Servaes G Ducreux |
| |
Institution: | (1) Morphogenèse Végétale Expérimentale, Université Paris Sud, Bat, 360, 91405 Orsay Cedex, France;(2) Seameo - Biotrop, Jl Raya Tajur Km 6, P.O. Box 116, Bogor, Indonesia;(3) Projet FAO - CIAM, Laboratoire de culture in vitro, BP 2183, Libreville, Gabon |
| |
Abstract: | The application of new techniques for improvement of sweet potato crops, particularly including the exploitation of somaclonal
variation, gene transfer by genetic transformation and somatic hybridization, requires the control of plant regeneration from
tissue cultures. Shoots can easily be regenerated from explants of stems, petioles, leaves and roots, while callus cultures
do not produce any shoots. The potential of somatic embryogenesis and plant regeneration via embryogenesis was evaluated for
10 cultivars of sweet potato. Protocols for plant regeneration from cultured protoplasts have also been developed. Since mesophyll
was resistant to enzyme digestion, fragments of stems and petioles, callus and cell suspensions were used as source of protoplasts
of sweet potato. Series of transfers of protoplast-derived calluses, particularly those which had been obtained from in vitro
plants, to media containing a high level of zeatin resulted in successful formation of shoots in only two sweet potato cultivars.
In addition, the embryogenic potential was irreversibly lost through protoplast culture, since protoplasts isolated from embryogenic
cell suspensions developed into non-embryogenic callus. Consequently, an alternative protocol is being successfully developed
to improve plant regeneration from cultured protoplasts of sweet potato, involving first root formation from which shoots
can then be regenerated. Preliminary evaluation in field conditions in Gabon revealed that plants regenerated from cultured
protoplasts exhibited a great genetic variability in their growth and tuber formation in particular.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | Sweet potato Ipomoea batatas plant regeneration somatic embryogenesis protoplasts genetic variation somaclonal variation |
本文献已被 SpringerLink 等数据库收录! |
|