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鸭肝炎病毒河南株的免疫保护试验研究
引用本文:陶海静,李华玮. 鸭肝炎病毒河南株的免疫保护试验研究[J]. 郑州牧业工程高等专科学校学报, 2014, 0(1): 16-18
作者姓名:陶海静  李华玮
作者单位:河南牧业经济学院,河南郑州450011
基金项目:河南省自然科学基金资助项目(2010B230010).
摘    要:
为探讨河南省部分地区鸭肝炎病免疫失败的原因,对分离到的鸭肝炎野毒株(LY株)在雏鸭、鸭胚及鸡胚上进行免疫保护试验、交叉中和试验。结果表明DH-Ⅰ型血清对该LY株的免疫保护指数较低,明显低于LY株血清的保护指数,表明该疫区部分鸭肝炎野毒株的抗原性已发生改变;交叉中和试验对该结果进行了进一步的验证;另采用LY株和DHV-I病毒疫苗联合制备鸭肝炎高免蛋黄抗体用于临床免疫防治可使临床保护率达到90%以上。

关 键 词:鸭肝炎  高免蛋黄  免疫保护试验  免疫保护率

Studies on the duck hepatitis-cross-protection test for field isolates strain from Henan
TAO Hai-jing,LI Hua-wei. Studies on the duck hepatitis-cross-protection test for field isolates strain from Henan[J]. Journal of Zhengzhou College of Animal Husbandry Engineering, 2014, 0(1): 16-18
Authors:TAO Hai-jing  LI Hua-wei
Affiliation:(Henan University of Animal Husbandry and Economy, Zhenzhou Henan 450011, China)
Abstract:
To explore the reason of immune failure for Henan around, the cross-protection tests and cross neutralization tests were conducted with the isolates strain of DHV From Luoyang(LYstrain). The results showed that the anti-genic variation could occur in LYstrain by cross-protection tests and cross-neutralization tests. Thereby a hyperimmue yolk antibody of the LYstrains of DHV-I and the standard strain of DHV-I was used in field tests, with the protective percentage to be superup to 90%
Keywords:duck hepatitis  virus  hyperimmue yolk antibody  cross- protection tests  protective index
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