芜菁花叶病毒两分离物的CP基因及HC-Pro基因的比较

芜菁花叶病毒杭州分离物(HZWT)和昆明分离物(YNQC)在7种寄主上的致病性相似.利用IC-RT-PCR对两分离物的CP和HC-Pro基因进行PCR扩增,扩增产物克隆后进行序列测定,CP基因序列长度均为864个核苷酸,编码288个氨基酸;HC-Pro基因序列长度均为1374个核苷酸,编码458个氨基酸.两分离物的CP和HC-Pro基因的核苷酸同源率分别为99.3%和94.5%,氨基酸同源率分别为100%和98.7%.HZWT和YNQC的CP基因与已报道的TuMV其他分离物的核苷酸序列同源率为89.2%～99.3%,氨基酸同源率分别为88.5%～99.7%;HC-Pro基因与TuMV其他分离物的核苷酸序列同源率分别为80.6%～97.0%,氨基酸同源率分别为95.0%～99.6%.

Comparisons of CP gene and HC-Pro gene of two Turnip mosaic virus isolates

The pathogenicity of Turnip mosaic virus(TuMV) isolates HZWT collected from Hangzhou and YNQC from Kunming were similar in tested seven hosts. CP and HC-Pro genes of two isolates were then amplified by IC-RT-PCR.The PCR products were cloned and determined.CP genes of the two isolates were all composed of 864 nucleotides encoding a polypeptide of 288 amino acids;HC-Pro genes of the two isolates were composed of 1374 nucleotides encoding a polypeptide of 458 amino acids.Nucleotide sequence identity of CP and HC-Pro genes between HZWT and YNQC were 99.3% and 94.5%,respectively,while amino acid identities of those between HZWT and YNQC were 100% and 98.7%,respectively.CP genes of HZWT and YNQC have 89.2%-99.3%(nucleotides) and 88.5%-99.7%(amino acids) sequence identities with other reported TuMV isolates,while HC-Pro genes of two isolates have 80.6%-97.0%(nucleotides) and 95.0%99.6%(amino acids) sequence identities with other reported TuMV isolates.