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| 牦牛LHB基因的分子克隆与序列分析 | |
| 引用本文： | 陈雪梅,字向东,余劲聪. 牦牛LHB基因的分子克隆与序列分析[J]. 家畜生态, 2008, 29(1): 31-33 |
| 作者姓名： | 陈雪梅 字向东 余劲聪 |
| 作者单位： | 西南民族大学动物科学国家民委重点实验室 四川成都610041(陈雪梅，字向东)，华侨大学工业生物技术福建省高等学校重点实验室 福建泉州362021(余劲聪) |
| 基金项目： | 四川省科技厅应用基础项目（05JY029-037-2）. |
| 摘 要： | 采用PCR法成功克隆出牦牛LHB基因的序列，在NCBI上的登录号为DQ508150。牦牛LHB基因的cds长426bp，编码141个氨基酸的产物。同源性分析，多个物种间LHB基因编码区的序列相似性在80％以上。通过牦牛与奶牛在LH的氨基酸序列上的比对发现，存在三处残基的差异，精氨酸-谷氨酰胺、甲硫氨酸-缬氨酸、苏氨酸-丙氨酸。 |
| 关 键 词： | 牦牛 促黄体素β亚基基因 系统发生树 雄性不育 |
Molecular Clone and Sequence Analysis of LHB Gene in Yak |
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| CHEN Xue-mei,ZI Xiang-dong,YU Jin-cong. Molecular Clone and Sequence Analysis of LHB Gene in Yak[J]. Ecology of Domestic Animal, 2008, 29(1): 31-33 | |
| Authors: | CHEN Xue-mei ZI Xiang-dong YU Jin-cong |
| Affiliation: | CHEN Xue-mei1,ZI Xiang-dong1,YU Jin-cong2 |
| Abstract: | The sequence of LHB gene in yak was cloned successfully by PCR method. The access number of this sequence in NCBI was DQ508150. The eels of LHB gene in yak was 426 bp long, coding a product with 141 amino acids. With analysis of homology, the similar degree of the eds of LHB gene among several species was more than 80 percent. With alignment of the amino acid sequences of LH β subunit between yak and cow, the difference of three amino acid residues was found, that is, Arg-Gln, Met-Val, Thr-Ala. |
| Keywords: | Yak Luteinizing hormone beta subunit gene Phylogenetic tree Male sterility |
| 本文献已被 CNKI 维普 等数据库收录！ | |