| NaN3诱变处理对苜蓿愈伤组织抗旱及盐生理的响应 |
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| 引用本文： | 李波,马赫.NaN3诱变处理对苜蓿愈伤组织抗旱及盐生理的响应[J].种子,2017(8):47-51. |
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| 作者姓名： | 李波 马赫 |
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| 作者单位： | 齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省应用技术研究与开发计划重大项目(GA 15 B 105-5)，齐齐哈尔市科学技术计划项目(NYGG-201518) |
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| 摘 要： | 采用植物组织培养和叠氮化钠(NaN3)诱变方法,对2种苜蓿愈伤组织进行诱变和NaC1、PEG、混合胁迫处理,测定NaN3诱变对苜蓿愈伤组织5项生理指标的变化.结果表明:随着NaN3诱变和胁迫处理浓度的增加,苜蓿愈伤组织的可溶性糖、可溶性蛋白和脯氨酸含量均呈上升的趋势,电导率和丙二醛含量呈下降的趋势,NaN3诱变+混合胁迫处理可溶性糖、可溶性蛋白和脯氨酸含量积累最多,电导率和丙二醛含量最少.
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| 关 键 词： | 紫花苜蓿 愈伤组织 NaN3 旱和盐胁迫 生理指标 |
Response of Physiology to Drought and Salt Tolerance of Alfalfa Callus by NaN3 Mutation |
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| LI Bo,MA He.Response of Physiology to Drought and Salt Tolerance of Alfalfa Callus by NaN3 Mutation[J].Seed,2017(8):47-51. |
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| Authors: | LI Bo MA He |
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| Abstract: | Experiment the method of plant tissue culture and NaN3 mutagenesis were adopted to induced and treated with hybrid stress of NaC1 and PEG the two kinds of alfalfa callus,Alfalfa callus five changes of physiological indexes were determined under NaN3 mutagenesis.The results showed that with the increase of ultraviolet mutagenesis and stress alfalfa,callus of soluble sugar,soluble protein and proline content present a rise of change trend,conductivity and malondialdehyde content showed a downward trend.NaN3 mutagenesis+hybrid stress dealing with soluble sugar,accumulation of soluble protein and proline was most,conductivity and malondialdehyde content was least. |
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| Keywords: | alfalfa callus NaN3 drought and salt stress physiological index |
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