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| 贵州马铃薯引进品种SSR分子标记及遗传多样性分析 | |
| 引用本文： | 李恩宏,贾长城,杨丽娜,滕安平,冯浪,朱英,李飞. 贵州马铃薯引进品种SSR分子标记及遗传多样性分析[J]. 种子, 2017, 0(7): 25-29. DOI: 10.16590/j.cnki.1001-4705.2017.07.025 |
| 作者姓名： | 李恩宏 贾长城 杨丽娜 滕安平 冯浪 朱英 李飞 |
| 作者单位： | 1. 贵州省种子管理站,贵阳,550001;2. 贵州大学,贵阳,550025;3. 贵州省生物技术研究所,贵阳,550006 |
| 基金项目： | 抗晚疫病马铃薯品种选育(黔科合NZ字[2012]3034)，马铃薯高产多抗新品种选育(黔农科院院专项[2014]033号) |
| 摘 要： | 为了鉴定马铃薯品种间的亲缘关系,采用5对SSR分子标记引物,对18个贵州马铃薯生产品种进行SSR分子标记及遗传多样性分析.结果表明:5对SSR引物共扩增出77个多态性条带,每个组合的多态性条带数为10～24不等,平均每个引物组合产生15.4个多态性条带.18个马铃薯材料之间的遗传距离范围在0.376 6～0.909 0之间,平均为0.701 1.经聚类分析,18个马铃薯材料在遗传距离0.57水平上全部聚为一类,以遗传距离0.60为基准,可明显聚为4个类群.第Ⅰ类包括5个材料;第Ⅱ类仅1个材料;第Ⅲ类包括4个材料;第Ⅳ类包括8个材料. |
| 关 键 词： | 马铃薯 品种 标记 遗传多样性 |
SSR Molecular Markers and Genetic Diversity Analysis of Potato Introduced Varieties in Guizhou |
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| LI Enhong,JIA Changcheng,YANG Lina,TENG Anping,FENG Lang,ZHU Ying,LI Fei. SSR Molecular Markers and Genetic Diversity Analysis of Potato Introduced Varieties in Guizhou[J]. Seed, 2017, 0(7): 25-29. DOI: 10.16590/j.cnki.1001-4705.2017.07.025 | |
| Authors: | LI Enhong JIA Changcheng YANG Lina TENG Anping FENG Lang ZHU Ying LI Fei |
| Abstract: | In order to identify the genetic relationship among potato cultivars,genetic diversity of 18 Potato Cultivars in Guizhou were analyzed by 5 pairs of SSR molecular marker primers.The results showed that 77 polymorphic bands were amplified by 5 pairs of SSR primers,and the number of polymorphic bands in each combination was 10-24.There were 15.4 polymorphic bands in each primer combination.The genetic distances between 18 potato materials ranged from 0.376 6 to 0.909 0,with an average of 0.701 1.By cluster analysis,18 potato samples were clustered into 4 groups at the 0.57 level of genetic distance.Based on the genetic distance of 0.60,they could be clustered into two groups.Category Ⅰ consists of 5 cultivars;Category Ⅱ only has 1 cultivar;Category Ⅲ consists of 4 cultivars;Category Ⅳ includes 8 cultivars. |
| Keywords: | potato variety marker genetic diversity |
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