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稀释方法对绒山羊冷冻精液品质的影响
引用本文:路佩瑶,任有蛇,申慧敏,岳文斌,姚继广,杨子森,王慧生,赵兴财,白海全. 稀释方法对绒山羊冷冻精液品质的影响[J]. 中国畜牧兽医, 2009, 36(6): 101-103
作者姓名:路佩瑶  任有蛇  申慧敏  岳文斌  姚继广  杨子森  王慧生  赵兴财  白海全
作者单位:(1.山西农业大学动物科技学院, 太谷 030801; 2.山西省生态畜牧产业管理站, 太原 030001;3.岢岚县畜牧兽医局, 岢岚 036300; 4.岢岚县绒山羊种羊场, 岢岚 036300)
基金项目:国家"十一五"科技支撑计划项目,山西省青年科学基金项目 
摘    要:选取7只精液品质优良的雁门绒山羊种公羊,通过测定其冷冻精液解冻后的精子活率(0、4 h)、精子存活指数、顶体完整率及精清中透明质酸酶(HYD)、谷草转氨酶(GOT)、乳酸脱氢酶(LDH)酶活力,研究一步稀释法和二步稀释法对冷冻精液品质的影响。结果表明,二步稀释法解冻后的精子存活指数显著(P<0.05)高于一步稀释法,其解冻后精清中透明质酸酶、谷草转氨酶、乳酸脱氢酶的活力均显著(P<0.05)低于一步稀释法。表明采用二步稀释法冷冻的绒山羊精液品质好于一步稀释法。

关 键 词:绒山羊  稀释方法  冷冻精液  

Effects of Dilution Methods on Frozen Semen Quality of Cashmere Goats
LU Pei-yao,REN You-she,SHEN Hui-min,YUE Wen-bin,YAO Ji-guang,YANG Zi-sen,WANG Hui-sheng,ZHAO Xing-cai,BAI Hai-quan. Effects of Dilution Methods on Frozen Semen Quality of Cashmere Goats[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(6): 101-103
Authors:LU Pei-yao  REN You-she  SHEN Hui-min  YUE Wen-bin  YAO Ji-guang  YANG Zi-sen  WANG Hui-sheng  ZHAO Xing-cai  BAI Hai-quan
Affiliation:(1.College of Animal Science and Technology,Shanxi Agricultural University, Taigu 030801, China; 2.Shanxi Ecological Animal Husbandry Industry Management Center, Taiyuan 030001, China;3.Kelan Animal Husbandry and Veterinary Bureau, Kelan 036300, China; 4.Kelan Cashmere Goat Breeding Farm, Kelan 036300, China)
Abstract:Seven cashmere goats producing great semen was selected.Sperm motility(0,4 h),sperm survival index,acrosomal integrity,enzyme activity of HYD,GOT and LDH after frozen-thawed were measured,to study the effect of one-step dilution method and two-step dilution method on semen freezing and frozen-thawed spermatozoa quality.The results showed that sperm survival index of two-step dilution method was higer significantly than that of one-step dilution method after frozen-thawed.Enzyme activity of HYD,GOT and LDH o...
Keywords:cashmere goats  dilution method  frozen semen  
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