Leptin对猪成肌细胞中PGC-1α、UCPs mRNA表达的影响

摘要：本实验采用链酶蛋白酶消化法获得猪成肌细胞， MyoG和MyoD的RT-PCR和Desmin免疫组化染色鉴定猪成肌卫星细胞后，分别研究外源leptin对猪成肌细胞产热相关基因PGC-1α和 UCPs mRNA表达的影响。对产热相关基因的RT-PCR检测结果表明，30 nmol/L 和100nmol/LLeptin均可促进成肌细胞中PGC－1αmRNA表达，100nmol/L Leptin作用效果显著高于30 nmol/L，且作用24h促进效果明显优于12h作用效果(p<0.05)。30nmol/LLeptin可明显提高成肌细胞中UCP2mRNA的表达，且作用24h促进效果明显优于12h处理效果(p<0.05)， 100nmol/L leptin作用效果显著低于30nmol/L Leptin作用效果，但显著高于对照组(p<0.05)。30 nmol/L 和100nmol/L leptin均不影响 UCP3mRNA的表达(p<0.05)。以上研究结果提示，Leptin可能通过上调PGC-1α和UCP2转录，促进成肌细胞能量消耗。

Effects of Leptin on the expression of PGC-1α、UCPs mRNA in pig myoblasts

Abstract: Pig myoblasts were obtained by pronase digestion and identified by Desmin immunohistochemistry and the mRNA expression of MyoD and MyoG. The effects of leptin on the expression of thermogenesis-related genes PGC-1αand UCPs were determined by RT- PCR. It was showed that 30 nmol/L and 100nmol/L leptin promote the mRNA expression of PGC-1αin myoblasts. The effect of 100nmol/L Leptin treated for 24h was significantly higher than that of 30 nmol/L Leptin for 12h(p<0.05). Moreover, 30 nmol/L leptin markedly promoted the UCP2 mRNA expression of myoblasts, and it was higher when treated for 24h than that of 12h (p<0.05). The effect 100nmol/L leptin on UCP2 mRNA expression was significantly lower than that of 30 nmol/L Leptin in 12h and 24h, but markedly higher than that of control(p<0.05). Additionally, the mRNA expression of UCP3 was not significantly different when treated by 30 nmol/L and 100nmol/L leptin compared to control in myoblasts(p<0.05). It suggests that leptin stimulate energy consumption by promoting the mRNA expression of PGC-1αand UCP2 in myoblasts.