Soybean oil content QTL mapping and integrating with meta-analysis method for mining genes |
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Authors: | Zhao-ming Qi Qiong Wu Xue Han Ya-nan Sun Xiang-yu Du Chun-yan Liu Hong-wei Jiang Guo-hua Hu Qing-shan Chen |
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Institution: | (1) Northeast Agricultural University, Harbin, China;(2) Research Center for Crop Breeding of Hei-Long-jiang Land Reclamation, Harbin, China; |
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Abstract: | Oil content of soybean was a valuable quantitative trait controlled by multiple genes. Eleven QTLs were detected by both CIM
and MIM method with the population crossed between Charleston and Dong nong594 in recent 3 years (2007, 2008, 2009). Combining
the QTLs collected over the past 20 years, an integrated map of oil-content major QTLs in soybean was established using soymap2,
which was published in 2004, as a reference. Using the software BioMercator ver.2.1, QTLs were projected from their own maps
onto the reference map. In total, ninety-eight QTLs were integrated into soymap2. A meta-analysis method was used to narrow
down the confidence interval, and 20 consensus QTLs and their corresponding markers were obtained. Using a local version of
GENSCAN, 10,137 sequences in the consensus QTL intervals were predicted. With BLAST, these predicted genes were compared to
the International Protein Index database to mine the related genes. The results offer a basis for gene mining and molecular
breeding in soybean. |
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