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| 爪哇稻Wanilava细胞质雄性不育系的创建及AFLP指纹图谱分析 | |
| 引用本文： | 陈永军,谢崇华,张景荣,郑文静,胡尚连,魏炘,王琦琳,杞从婷,刘永胜.爪哇稻Wanilava细胞质雄性不育系的创建及AFLP指纹图谱分析[J].中国水稻科学,2008,22(1):28-32. |
| 作者姓名： | 陈永军 谢崇华 张景荣 郑文静 胡尚连 魏炘 王琦琳 杞从婷 刘永胜 |
| 作者单位： | 1. 西南科技大学,生命科学与工程学院,四川,绵阳,621002 2. 四川大学,生命科学院,生物资源与生态环境教育部重点实验室,四川,成都,610064 3. 西南科技大学,生命科学与工程学院,四川,绵阳,621002;四川大学,生命科学院,生物资源与生态环境教育部重点实验室,四川,成都,610064 |
| 基金项目： | 四川省“十一五”水稻育种攻关资助项目（2006-YZGG1）. |
| 摘 要： | 初步鉴定了爪哇稻Wanilava细胞质雄性不育系的特征特性，并以2套水稻（Oryza sativa）同核异质材料273JW（爪哇型胞质不育系）、273D（D型胞质不育系）及其对应的保持系273B， 803JW（爪哇型胞质不育系）、803WA（野败型胞质不育系）及其对应的保持系803B为研究对象，利用AFLP 分子标记技术构建了JW型不育胞质的指纹图谱，分离了JW型胞质与野败型胞质及保持系的差异片段。 |
| 关 键 词： | 细胞质雄性不育 同核异质材料 扩增片段长度多态性 |
| 文章编号： | 1001-7216（2008）01-0028-05 |
| 收稿时间： | 2007-06-18 |
| 修稿时间： | 2007-09-19 |
Breeding of Cytoplasmic Male Sterile Line Derived from Oryza sativa subsp, javanica cv.Wanilava and Its AFLP Fingerprints |
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| CHEN Yong-jun,XIE Chong-hua,ZHANG Jing-rong,ZHENG Wen-jing,HU Shang-lian,WEI Xin,WANG Qi-lin,QI Cong-ting,LIU Yong-sheng.Breeding of Cytoplasmic Male Sterile Line Derived from Oryza sativa subsp, javanica cv.Wanilava and Its AFLP Fingerprints[J].Chinese Journal of Rice Science,2008,22(1):28-32. | |
| Authors: | CHEN Yong-jun XIE Chong-hua ZHANG Jing-rong ZHENG Wen-jing HU Shang-lian WEI Xin WANG Qi-lin QI Cong-ting LIU Yong-sheng |
| Abstract: | The characteristics of a novel cytoplasmic male sterile (CMS) line derived from Oryza sativa subsp. javanica cv. Wanilava (denominated as JW type) were analyzed. Two sets of isonucleus allocytoplasmic lines (273D, 273JW and 273B； 803WA, 803JW and 803B, respectively) were bred by advanced backcrosses using the maintainer lines (273B and 803B, respectively) as recurrent parents, each containing individual cognate nucleus with different cytoplasms. Considerable genetic diversities between CMS JW (CMS from javanica cv. Wanilava) and CMS WA (CMS of wild abortive from O. rufipogon) or CMS D (CMS from African indica cv. Dissi) were revealed by AFLP. The differential fragments from the cytoplasms were isolated. |
| Keywords: | cytoplasmic male sterility isonueleus allocytoplasmic lines amplified fragment length polymorphism fingerprint javanica rice |
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