| 一例鸡大肠杆菌病的病原分离鉴定 |
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| 引用本文： | 李乔斌,谢晓东,陈静,尹德晶,杨鹏,程振涛,岳筠.一例鸡大肠杆菌病的病原分离鉴定[J].贵州畜牧兽医,2020,44(1):50-53. |
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| 作者姓名： | 李乔斌 谢晓东 陈静 尹德晶 杨鹏 程振涛 岳筠 |
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| 作者单位： | 贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025;贵州省动物疫病研究室,贵州 贵阳 550025,贵州省动物疫病预防控制中心,贵州 贵阳 550008 |
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| 基金项目： | "贵州省动物生物制品工程技术研究中心"贵州省科技平台及人才团队计划;"贵州省预防兽医专业硕士研究生工作站"贵州省研究生教育创新计划项目 |
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| 摘 要： | 为确诊某养鸡场发病的原因,采集临床病死鸡的肝脏、脾脏进行细菌分离培养,并通过细菌形态学观察、生化试验、分子生物学鉴定和药物敏感性试验.结果:分离细菌为革兰氏阴性杆菌,菌落形态和生化鉴定特征与大肠杆菌相符;16S rDNA PCR扩增及基因同源性分析确定为大肠杆菌;菌株对安普霉素、泰妙菌素、多西环素、硫酸粘菌素较敏感,对替米考星、泰万菌素、磺胺间甲氧嘧啶、泰乐菌素、阿莫西林存在耐药性.
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| 关 键 词： | 鸡 大肠杆菌病 细菌分离培养 PCR扩增 基因序列分析 药敏试验 |
Isolation and Identification of Pathogeny from A Case of Chicken Colibacillosis |
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| Li Qiaobin,Xie Xiaodong,Chen Jing,Ying Dejing,Yang Peng,Cheng Zhentao,Yue Jun.Isolation and Identification of Pathogeny from A Case of Chicken Colibacillosis[J].Guizhou Animal Science and Veterinary Medicine,2020,44(1):50-53. |
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| Authors: | Li Qiaobin Xie Xiaodong Chen Jing Ying Dejing Yang Peng Cheng Zhentao Yue Jun |
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| Institution: | (College of Animal Science,Guizhou University,Guiyang Guizhou 550025,China;Animal Epidemic Disease Research Office of Guizhou Province,Guiyang Guizhou 550025,China;Animal Disease Prevention and Control Center in Guizhou Province,Guiyang Guizhou 550008,China) |
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| Abstract: | In order to diagnose the cause of the disease in a chicken farm,the liver and spleen of clinically sick and dead chickens were collected for bacterial isolation and culture,and bacteria morphological observation,biochemical test,molecular biological identification and drug sensitivity test were conducted.Results:the isolated bacteria were gram-negative bacilli,and the colony morphology and biochemical characteristics were consistent with Escherichia coli.The 16S rDNA PCR amplification and gene homology analysis were confirmed as Escherichia coli.The strain was more sensitive to Apra-mycin,tiamulin,doxycycline and colistin sulfate,but had resistance to tilmicosin,Acetylisovaleryltylosin Tartrate,Sulfamonomethoxine,tylosin and amoxicillin. |
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| Keywords: | Chicken Colibacillosis Bacterial Isolation and Culture PCR Amplification Gene Sequence Analysis Drug Sensitive Test |
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