| 陕西杨陵葡萄黑痘病病原菌的分离与鉴定 |
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| 引用本文： | 肖欢,朱明旗,侯鸿敏,赵素平,王西平.陕西杨陵葡萄黑痘病病原菌的分离与鉴定[J].西北林学院学报,2010,25(2):132-135. |
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| 作者姓名： | 肖欢 朱明旗 侯鸿敏 赵素平 王西平 |
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| 作者单位： | 西北农林科技大学园艺学院农业部西北园艺植物种质资源与应用重点开放实验室;西北农林科技大学植保学院; |
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| 基金项目： | 国家自然科学基金，教育部新世纪优秀人才支持计划及陕西省自然科学研究项目 |
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| 摘 要： | 采用常规组织法对陕西杨陵葡萄黑痘病病原菌进行分离、纯化,共获得病原菌菌株24个,并对菌株YL018进行形态学鉴定、致病性测定及病原菌DNA同源性分析。利用ITS区域通用引物ITS1-F/ITS4进行病原菌DNA PCR扩增,将获得的病原菌rDNA-ITS区域的DNA序列进行Blast比较分析。结果发现,菌株YL018与GenBank登录号分别为AY826763、AY826762和AY826764的Elsinoe ampelina位于一个分支上。从而证明分离到了陕西杨陵葡萄黑痘病病原菌。也为进一步研究在病原菌侵染葡萄过程中,植物防御系统中酶类物质的变化及作用做好了前期基础。
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| 关 键 词： | 陕西杨陵 葡萄 黑痘病 分离 鉴定 |
Isolation and Identification of Elsinoe ampelina in Yangling |
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| XIAO Huan,ZHU Ming-qi,HOU Hong-min,ZHAO Su-ping,WANG Xi-ping.Isolation and Identification of Elsinoe ampelina in Yangling[J].Journal of Northwest Forestry University,2010,25(2):132-135. |
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| Authors: | XIAO Huan ZHU Ming-qi HOU Hong-min ZHAO Su-ping WANG Xi-ping |
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| Institution: | XIAO Huan1,ZHU Ming-qi2,HOU Hong-min1,ZHAO Su-ping1,WANG Xi-ping1(1.College of Horticulture,Northwest A&F University,Key Laboratory of Northwest Horticulture Plant Germplasm , Application of Agriculture Ministry,Yangling,Shaanxi 712100,China,2.College of Plant Protection,China) |
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| Abstract: | The pathogens of Elsinoe ampelina were isolated and purified from susceptible disease leaves of grape cultivar cultivated in Yangling,Shaanxi,China by the conventional tissue method.The total 24 strains of E.ampelina were obtained,and the strain YL108 was identified as the pathogens of E.ampelina through pathogenicity determination,morphology observation and analysis of nucleotide sequence of internal transcribed spacer(ITS).The fungal DNA sequence in rDNA-ITS region of YL108 shared highly identity with oth... |
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| Keywords: | Yangling Shaanxi grape anthracnose isolation identification |
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