| 哈尔滨地区猪、犬旋毛虫同工酶电泳比较的研究 |
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| 引用本文： | 宋铭忻,周源昌.哈尔滨地区猪、犬旋毛虫同工酶电泳比较的研究[J].东北农业大学学报,1994,25(3):260-265. |
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| 作者姓名： | 宋铭忻 周源昌 |
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| 作者单位： | 东北农业大学动物医学系 |
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| 摘 要： | 通过对猪、犬旋毛虫同工酶酶谱的研究发现，乳酸脱氢酶（ＬＤＨ）不能区别猪、犬被毛虫，而葡萄糖磷酸异构酶（ＧＰＩ）、苹果酸脱氢酶（ＭＤＨ）、苹果酸酶（ＭＥ）和磷酸葡萄糖转位酶（ＰＧＭ）可将猪、犬旋毛虫区分开来．猪、犬旋毛虫之间同工酶相似系数（Ｃ．Ｓ．）为２６．１％．试验结果揭示，哈尔滨地区的猪旋毛虫相当于旋毛形线虫（Ｔ．ｓｐｉｒａｌｉｓ），而犬旋毛虫相当于本地毛形线虫（试泽）（Ｔ．ｎａｔｉｖａ）．
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| 关 键 词： | 哈尔滨，猪，犬，旋毛虫，同工酶 |
COMPARATIVE STUDY ON ISOZYME PATTERNS OFTRICHINELLA SP.FROM SWINE AND DOGS INHARBIN REGION,CHINA |
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| Song Mingxin,Zhou Yuanchang and Li Shusheng.COMPARATIVE STUDY ON ISOZYME PATTERNS OFTRICHINELLA SP.FROM SWINE AND DOGS INHARBIN REGION,CHINA[J].Journal of Northeast Agricultural University,1994,25(3):260-265. |
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| Authors: | Song Mingxin Zhou Yuanchang and Li Shusheng |
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| Abstract: | Examination of isozyme by isoelectric focusing clectrophoresis in thin layerpolyacrylamide gel had revcaled differences between Trichinella sp. from twine and dogs inHarbin,China. Lactate dehydrogenase(LDH,EC 1.1,1 .27) gave no discrete clectrophoreticbands between Trichinella sp.from swine and dogs.However , glucose phosphate isomerase(GPI,EC 5.3.1.8),malate dehydrogenase (MDH, EC 1.1.1.37),malic enzyme (ME,EC1,1.1.40)and phosphoglucomutase(PGM,EC 2.7.5.1) distinguished between Trichinella sp.from swine and dogs, Coefficients or similarity between Trichinella sp. from swine anddogs was 26.k%.It was concluded that Trichinella sp . from swine and dogs in Harbin,China most probably corresponded.to the north temperate isolate (T. spiralls ) and arcticisolate (T. nativa) respcctively. |
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| Keywords: | Trichinella sp isozyme |
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