广西水稻地方品种核心种质矿质元素含量差异及相关分析

以419份广西水稻地方品种核心种质的糙米为材料,分别于2014和2015年测定了Ca、Fe、Zn和Se 4种矿质元素含量,同时利用34个SSR标记对两年矿质元素含量进行Pearson相关分析。结果表明：4种矿质元素在两年间的鉴定结果存在极显著差异,呈显著正相关。籼稻的Ca、Se平均含量高于粳稻,Ca（2014）、Se（2014）在籼粳稻间呈显著性差异;粳稻的Fe、Zn平均含量高于籼稻,Fe（2015）、Zn（2014,2015）在籼粳间呈显著性差异。矿质元素含量与稻作区经纬度、海拔等因素相关,Fe平均含量在桂南稻作区最高,Zn平均含量在桂中稻作区最高;Ca、Fe、Zn平均含量在黑米中含量最高,Se在红米中含量最高;Ca和Zn平均含量在黑米与红米、黑米与白米之间存在显著差异,Fe无显著差异。4种矿质元素含量在2年间的相关系数范围为0.159~0.557,均呈极显著正相关。2014年4种元素含量间无显著性相关;2015年Se和Fe、Zn之间呈显著正相关（P<0.05）,Zn和Fe之间显著正相关（P<0.01）,Zn和Ca之间极显著正相关（P<0.001）。SSR标记共检测到167个等位基因,平均4.91个,遗传多样性指数为0.574,遗传信息量为0.522。经Pearson相关分析,4种矿质元素共关联到18个相关位点,其中8个位点与前人的研究结果不相同。

Variation and Correlation Analysis of Mineral Elements Contents for Core Collection of Landrace Rice in Guangxi

The content of Ca, Fe, Zn and Se in brown rice of 419 core collection accessions of Guangxi landrace was analyzed in 2014 and 2015. A total of 34 SSR markers were used to identify the content of four mineral elements by Pearson correlation analysis in the two years. The content of the four mineral elements in the two years was significantly different and positively correlated. The content of Ca and Se in indica was higher than that in japonica with significant difference. The content of Fe and Zn in japonica was higher than that in indica with significant difference. The content of mineral elements was associated with longitude, latitude and altitude of the rice cropping regions. The content of Fe was the highest in southern Guangxi, that of Zn was the highest in central Guangxi, that of Ca, Fe, Zn was the highest in black rice, that of Se was the highest in red rice, that of Ca and Zn of black rice was significantly higher than that of red and white rice, and that of Fe was not significantly among three pericarp colors. The range of correlation coefficient of the content of the four mineral elements between the two years from 0.159 to 0.557, 2014 was significantly positively associated with 2015. There was no obvious difference among the content of the four elements in 2014. In 2015, the content of Se was significantly positively correlated with that of Fe and Zn (P<0.05), the content of Zn was significantly positively associated with that of Fe (P<0.01) and Ca (P<0.001). A total of 167 alleles were detected by SSR markers, with genetic diversity index 0.574 and genetic information 0.522. Pearson correlation analysis showed that 18 loci were associated to the content of the four mineral elements, among them, eight loci weree different from the previous studies. The study would provide a theoretical basis and material foundation for related gene mining and rice breeding with high mineral element content.