| 澳洲鸽子石斛兰花梗芽组织培养技术研究 |
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| 引用本文： | 陈和明,江南,吕复兵,肖文芳,李佐,朱根发.澳洲鸽子石斛兰花梗芽组织培养技术研究[J].热带作物学报,2020,41(8):1529-1534. |
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| 作者姓名： | 陈和明 江南 吕复兵 肖文芳 李佐 朱根发 |
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| 作者单位： | 1.广东省农业科学院环境园艺研究所,广东广州 5106402.广东省园林花卉种质创新综合利用重点实验室,广东广州 5106403.东莞市农业科学研究中心,广东东莞 523000 |
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| 基金项目： | 广东省重点领域研发计划项目(2018B020202001);东莞市社会科技发展项目(2016108101001);广东省现代农业产业技术体系创新团队项目(2019KJ121) |
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| 摘 要： | 以澳洲鸽子石斛兰（Dendrobium kingianum Bidwill）的花梗为外植体,研究花梗芽的诱导、增殖和生根情况。结果表明：在1号诱导培养基MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+10%椰子汁（CM）]和2号诱导培养基（MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+AC 1.0 g/L+10% CM）中均能诱导出芽,尽管在诱导过程中70.6%带节间的花梗茎段因不能诱导出侧芽或侧芽弱小而死亡,但为种苗生产和种质资源的保护提供了一种有效途径。在增殖培养过程中,2号增殖培养基（MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM）有利于增殖培养;在生根壮苗过程中,生根培养基（1/2 MS+NAA 0.3~0.5 mg/L+10% CM）适宜澳洲鸽子石斛兰‘金斯卡’的生根培养。
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| 关 键 词： | 澳洲鸽子石斛兰 花梗 组织培养 诱导 增殖 壮苗生根 |
| 收稿时间： | 2019-11-02 |
Tissue Culture Technology for Pedicel Shoots of Dendrobium kingianum Bidwill |
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| CHEN Heming,JIANG Nan,LYU Fubing,XIAO Wenfang,LI Zuo,ZHU Genfa.Tissue Culture Technology for Pedicel Shoots of Dendrobium kingianum Bidwill[J].Chinese Journal of Tropical Crops,2020,41(8):1529-1534. |
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| Authors: | CHEN Heming JIANG Nan LYU Fubing XIAO Wenfang LI Zuo ZHU Genfa |
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| Institution: | 1. Floricultural Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China2. Guangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Guangzhou, Guangdong 510640, China3. Dongguan Agricultural Science Research Center, Dongguan, Guangdong 523000, China |
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| Abstract: | The induction and proliferation and rooting tests of Dendrobium kingianum Bidwill were studied using pedicel shoots as the explant. The two induction media of MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+10% CM and MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+AC 1.0 g/L+10% CM could induce buds. 70.6% pedicels died without lateral buds induced or lateral buds too weak, but it provided an effective way for seedling production and germplasm resources protection. In the proliferation culture, the medium of MS+6-BA 3.0 mg/L+AD 3.0 mg/L+10% CM was beneficial to proliferation culture. In the rooting and growth culture, the medium of 1/2 MS+NAA 0.3-0.5 mg/L+10% CM was suitable for the rooting culture of D. kingianum ‘Jinsika’ (DJ). |
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| Keywords: | Dendrobium kingianum Bidwill pedicel tissue culture induction proliferation rooting |
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