<Emphasis Type="Italic">Verticillium</Emphasis> disease of <Emphasis Type="Italic">Agaricus bisporus</Emphasis>: variations in host contribution to total fungal DNA in relation to symptom heterogeneity |
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Authors: | Michèle L Largeteau Catherine Regnault-Roger Jean-Michel Savoie |
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Institution: | (1) INRA (Institut National de la Recherche Agronomique), UPR1264 MYCSA, 33883 Villenave d’Ornon, France;(2) UPPA (Université Pau et Pays de l’Adour), UFR Sciences et Techniques, 64012 Pau Universite Cedex, France |
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Abstract: | The pathogenic fungus Verticillium fungicola, responsible for dry bubble disease of the common mushroom Agaricus bisporus, causes various symptoms on its host, bubbles (undifferentiated spherical masses), bent and/or split stipes (blowout) and
spotty caps. Host DNA quantification by real-time PCR was used to observed relationships between the type of symptom and the
relative amount of A. bisporus and V. fungicola in diseased mushrooms. Verticillium fungicola is involved in bubble formation but does not appear to regulate its growth. Quantifications in bubbles and stipe-bubbles
(morphology between bubble and sporophore with stipe blowout) showed that the pathogen has no effect on the growth of undifferentiated
host hyphae but prevents morphological differentiation if not initiated and stops it when initiated hyphae are affected. Mushrooms
with stipe blowout exhibiting both mature and abortive lamellae reveal that V. fungicola has a restricted area of action in host tissues. Despite their visual aspect, healthy looking parts of mushrooms showing
spots or stipe blowout were actually contaminated. Discolouration and symptom development are two distinct events. The colour
of the tissues was correlated to the percentage of A. bisporus DNA, suggesting that discolouration is not an efficient defensive mechanism, and occurs at the time V. fungicola developed enough to induce tissues necrosis. |
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Keywords: | Mushroom Discolouration Dry bubble Host DNA detection Verticillium fungicola Real-time PCR |
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