巴西橡胶树核糖体蛋白S5基因的克隆及生物信息学分析

核糖体蛋白S5是核糖体蛋白家族的重要成员,在核糖体中发挥重要作用.利用本实验室获得的部分序列设计引物,采用3'-RACE技术从巴西橡胶树胶乳中获得一个核糖体蛋白S5基因的完整阅读框,该基因编码209个氨基酸的多肽,含有一个典型的真核生物核糖体蛋白S5结构域.在HbRPS5氨基酸序列中没有预测到跨膜区和信号肽,二级结构分析表明HbRPS5属于混合型蛋白.对14个RPS5系统进化分析表明,巴西橡胶树的RPS5基因在进化上具有保守性,与植物类拟南芥的RPS5基因亲缘关系最近,而与人和酵母等亲缘关系相对较远.该基因的克隆和生物信息学分析为深入研究其功能奠定了基础.

Cloning and Bioinformatic Analysis of the Gene Encoding the Ribosomal Protein S5 from Hevea brasiliensis

The ribosomal protein S5 is the key member of ribosomal protein family,and it plays an important role in the ribosomal proteins.The primers designed based on the known sequences,the gene was cloned with 3'-RACE technique from Hevea brasiliensis latex.The open reading frame encoded 209 amino acids,containing a conserved ribosomal S5 domain,so the gene was named as HbRRS5.No signal peptide and transmembrane structure were predicted within HbRPS5.The result of secondary structure predicted showed that the protein was classified as mixed type.The result of phylogenetic tree indicated that HbRPS5 displayed a high degree of sequence similarity to AtRPS5,but not to animals,such as Homo sapiens,Rattus norveegicus,and 80 on.This paper will be benefit to further analyze the functions of HbRPS5.