云南元江野生稻抽穗开花期耐热QTL定位

利用以籼稻(Oryza sativa ssp. indica) 品种特青为遗传背景的云南元江普通野生稻（O. rufipogon Griff.）(简称“元江普野”)渗入系为材料，调查温室高温胁迫条件下野生稻渗入系和受体亲本特青的结实率，采用单标记回归分析法，共检测到4个抽穗开花期耐热性相关的QTL，分别位于第1、3、8和10染色体。其中位于第1和3染色体上的2个QTL（qHT1和qHT3），贡献率分别为12%和6%，来自元江普野的等位基因能提高群体的耐热性，分别可增加9.13%和6.71%的结实率。而位于第8、10染色体上的2个QTL（qHT8和qHT10），贡献率均为6%，来自元江普野的等位基因降低群体的耐热性，加性效应分别为-6.44%和-4.44%。研究结果不仅为耐热水稻品种的分子标记辅助育种提供参考，而且充分显示了利用野生稻的优异基因改良栽培稻抗逆性状的巨大潜力。

Identification of QTLs Associated with Heat Tolerance of Yuanjiang Common Wild Rice (Oryza rufipogon Griff.) at Flowering Stage

Using an introgression lines population of Chinese common wild rice（O. rufipogon Griff.）from Yuanjiang, Yunnan Province, with the background of Teqing (O. sativa ssp. indica), Four quantitative trait loci (QTLs) for heat tolerance at the flowering stage were identified by the method of single-point regression analysis. Two QTLs (qHT1and qHT3) mapped on chromosome 1 and 3, explained 12% and 6% of the phenotypic variance, respectively, and the O.rufipogon-derived alleles contributed desired effects on Teqing background, which could increase 9.13% and 6.71% of percent seed set. The other two QTLs （qHT8 and qHT10）detected on chromosome 8 and 10, explained 6% and 6% of the phenotypic variance, respectively, and the O. rufipogon-derived alleles can decrease percent seed set on Teqing background. The results obtained in this study might be helpful for improvement of heat tolerance by marker-assisted selection in rice. It was also suggested that there was enormous potential for improving the tolerance to abiotic stress of cultivated rice utilizing the favorable genes of wild species in rice breeding program.