| 小麦DNA提取方法的比较 |
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| 引用本文： | 田再民,龚学臣,张利增. 小麦DNA提取方法的比较[J]. 湖北农业科学, 2009, 48(7) |
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| 作者姓名： | 田再民 龚学臣 张利增 |
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| 作者单位： | 河北北方学院农林科技学院,河北,张家口,075131;北京市农林科学院北京杂交小麦研究中心,北京,100097;河北北方学院农林科技学院,河北,张家口,075131;张家口市农业局,河北,张家口,075000 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，北京市农业育种基础研究创新平台项目 |
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| 摘 要： | 以小麦幼叶为材料,采用CTAB法1、CTAB法2、CTAB法3和NaOH法提取小麦基因组DNA,通过对小麦DNA提取方法的比较,为满足大批量材料进行SSR分子标记的检测寻找最优方法.结果表明,NaOH法提取DNA用时短、方法简单,但常有DNA样品扩增不出PCR产物的情况;CTAB法提取的DNA质量较高,而且CTAB法3省略了65℃温浴,并将两次氯仿:异戊醇抽提改为1次抽提,既缩短了试验时间和降低了成本,提取的DNA又可以满足进行分子标记检测的需要.
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| 关 键 词： | 小麦 DNA 提取 CTAB |
Comparison of Several Kinds of Wheat DNA Extraction Methods |
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| TIAN Zai-min,GONG Xue-chen,ZHANG Li-zeng. Comparison of Several Kinds of Wheat DNA Extraction Methods[J]. Hubei Agricultural Sciences, 2009, 48(7) |
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| Authors: | TIAN Zai-min GONG Xue-chen ZHANG Li-zeng |
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| Affiliation: | 1.College of Agriculture and Foresty;Hebei North University;Zhangjiakou 075131;Hebei;China;2.Beijing Research Center for Hybrid Wheat;Beijing Academy of Agricultural and Forestry Sciences;Beijing 100097;3.Agriculture Office of Zhangjiakou;Zhangjiakou 075000;China |
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| Abstract: | In order to find out a better wheat DNA extracting method for the molecular marker analysis of large quantity samples,wheat leaves were used as experimental materials to study the DNA extracting methods such as CTAB 1,CTAB 2,CTAB 3 and NaOH method.The results showed that the NaOH method was very simple,but couldn't produce PCR.CTAB method reduced the cost and saved time for the experiment,improved the quality of DNA and simplified the extracting steps.The experiment indicated that CTAB 3 extracting method c... |
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| Keywords: | DNA CTAB |
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