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Effect of Tissue Processing on Assessment of Endoscopic Intestinal Biopsies in Dogs and Cats |
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| Authors: | MD Willard GE Moore BD Denton MJ Day J Mansell T Bilzer B Wilcock M Gualtieri D Olivero P Lecoindre DC Twedt MG Collett EJ Hall AE Jergens JW Simpson RW Else RJ Washabau |
| Institution: | Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX;;Department of Comparative Pathobiology, Purdue University, West Lafayette, IN;;Division of Veterinary Pathology, Infection and Immunity, School of Clinical Veterinary Science, University of Bristol, Langford, UK;;Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX;;Institut fur Neuropathologie, Heinrich-Heine-Universitat Dusseldorf, Dusseldorf, Germany;;Histovet Surgical Pathology, Guelph, Ontario, Canada;;Sezione di Clinica Chirugica, Dipartimento di Scienze Cliniche Veterinarie, Faculty of Veterinary Medicine, University of Milan, Milano, Italy;;Laboratory Analysis BiESSEA, University of Milan, Milano, Italy;;Clinique Veterinaire des Cerisioz, 5 Rte De St Symphorien d'Ozon, St Priest, France;;Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO;;Pathobiology Group, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand;;Division of Companion Animal Studies, School of Clinical Veterinary Science, University of Bristol, Langford, UK;;Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA;;Royal (Dick) School of Veterinary Studies, University of Edinburgh, Veterinary Field Station, Roslin, Scotland, UK;;and Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN |
| Abstract: | Background: Prior studies failed to detect significant association between hypoalbuminemia and small intestinal lesions. Hypothesis: Use of pictorial templates will enhance consistency of interpathologist interpretation and identification of intestinal lesions associated with hypoalbuminemia. Animals: Tissues from 62 dogs and 25 cats examined as clinical cases at 7 referral veterinary practices in 4 countries. Methods: Retrospective, observational study. Histopathology slides from sequential cases undergoing endoscopic biopsy were examined by 4 pathologists by pictorial templates. Changes for 9 microscopic features were recorded as normal, mild, moderate or severe, and 2- and 4-point scales were tested for consistency of interpretation. Logistic regression models determined odds ratios (OR) of histologic lesions being associated with hypoalbuminemia while κ statistics determined agreement between pathologists on histologic lesions. Results: There was poor agreement (κ=?0.013 to 0.3) between pathologists, and institution of origin of slides had effect (κ= 1.0 for 3 of 4 lesions on slides from Institution 5) on agreement between pathologists on selected histologic features. Using 2 point as opposed to 4-point grading scale increased agreement between pathologists (maximum κ= 0.69 using 4-point scale versus maximum κ= 1.0 using 2-point scale). Significant association ( P = .019– .04; 95% OR = 3.14–10.84) between lacteal dilation and hypoalbuminemia was found by 3 pathologists. Conclusions and Clinical Importance: Substantial inconsistency between pathologists remains despite use of pictorial template because of differences in slide processing. Distinguishing between mild and moderate lesions might be important source of the disagreement among pathologists. |
| Keywords: | Histology Inflammatory bowel disease Protein-losing enteropathy |