油樟组织培养技术研究

以当年生油樟枝条为研究对象,探索了消毒剂种类、浓度及其作用时间、MS培养基、6-BA、NAA、IBA等5种因素对油樟茎段组织培养的影响,分析筛选出了一套能够产出具有优良素质油樟种苗的组织培养技术。结果表明:油樟茎段经10%84处理15min或15%84处理10 min两种方法进行消毒均能获得污染率<15%,且成活率>70%的无菌外植体;适合不定芽诱导的培养基为:蔗糖30 g·L^-1+卡拉胶6 g·L^-1+MS+6-BA 1.0 mg·L^-1+IBA 0.1 mg·L^-1;适合不定芽增殖继代的培养基:蔗糖30 g·L^-1+卡拉胶6 g·L^-1+MS+6-BA 1.0 mg·L^-1+NAA 0.10 mg·L^-1+IBA 0.2 mg·L^-1;生根培养基:蔗糖15 g·L^-1+卡拉胶6 g·L^-1+1/2MS+IBA 1.5 mg·L^-1+NAA 1.0 mg·L^-1。

A Study of Tissue Culture of Cinnamomum longepaniculatum

The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15 min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+ IBA 0.1 mg·L^-1;The medium for multiplication of adventitious buds was sucrose 30 g·L^-1+carrageenan 6 g·L^-1+MS+6-BA 1.0 mg·L^-1+NAA 0.10 mg·L^-1+IBA 0.2 mg·L^-1;The rooting medium was sucrose 15 g·L^-1+carrageenan 6 g·L^-1+1/2 MS+IBA 1.5 mg·L^-1+NAA 1.0 mg·L^-1.