川硬皮肿腿蜂学习经历对花椒与虎天牛虫粪挥发物的行为反应测定

采用Y型嗅觉仪和触角电位仪,研究羽化初期川硬皮肿腿蜂对花椒挥发物、花椒虎天牛虫粪木屑混合物诱导的学习效应,比较有无学习经历的川硬皮肿腿蜂对不同浓度花椒挥发物的行为反应,以探索利用学习行为提高川硬皮肿腿蜂防治花椒虎天牛效果的途径。结果表明:羽化期经历10g·mL-1花椒枝条挥发物诱导的川硬皮肿腿蜂对味源的行为反应率最高,达到66.67%,与其余9种处理间存在显著差异(P<0.05),其次是羽化期经历10g·mL-1虫粪木屑挥发物诱导的肿腿蜂,其对味源的行为反应率为64.44%,而经历0.01g·mL-1花椒枝条与虫粪木屑挥发物诱导的肿腿蜂反应率最低,表明经花椒挥发物和花椒虎天牛虫粪学习处理的川硬皮肿腿蜂对防治花椒虎天牛有正面影响,且花椒枝条挥发物在同类因素中对川硬皮肿腿蜂防治花椒虎天牛有最突出影响的结论。

Determination of Behavioral Responses of Scleroderma sichuanensis with Learning Experience to the Volatiles of Host and Feces of Clytus valiandus

By Y type olfactometer and electroantennogram, studies were made of the learning effect of Scleroderma Sichunensis in early emergence period to the in duction of pepper volatiles and mixture of Clytus valiandus feces and sawdust. The behavioral responses to different concentrations of volatiles were compared between S. sichunensis with learning experience and those without learning experience, which could be a way to improve the prevention efficiency of S.sichuanensis .The results showed that the the highest behavioral response rate of S.sichuanensis was 66.67%, induced by 10 g·mL -1 of pepper branches volatiles in emergence period,which were significantly different from the other 9 treatments (P<0.05). The second highest rate was 64.44% for S.sichuanensis induced by 10 g·mL -1 insect feces and sawdust volatile flavor. And the rate was lowest when S.sichuanensis was induced by 0.01 g·mL -1 L of pepper branches and insect feces sawdust volatile. The pepper volatiles and Clytus valiandus feces learning process for S.sichuanensis showed positive effect on prevention, and pepper shoots volatiles were the most prominent prevention effect.