| 百合组培快繁技术研究 |
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| 引用本文： | 王永江,张振臣,张丽芳,乔奇,靳秀兰.百合组培快繁技术研究[J].河南农业科学,2004(5):55-58. |
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| 作者姓名： | 王永江 张振臣 张丽芳 乔奇 靳秀兰 |
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| 作者单位： | 河南省农业科学院植物保护研究所,河南,郑州,450002 |
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| 基金项目： | 河南省农业科学院“十五”重点项目 (1 0 5 0 1 1 4 )，河南省科技攻关项目 (0 32 4 0 5 0 0 30 )资助 |
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| 摘 要： | 以食用百合鳞片作外植体 ,以MS为基本培养基 ,对其组培快繁技术进行了研究。结果表明 ,适宜的芽诱导培养基为MS + 1 .0mg/L 6-BA + 0 .1mg/LNAA + 1 .0mg/LKT ;适宜的快繁培养基为MS + 1 .0mg/L 6-BA + 0 .0 1mg/LNAA + 0 .5mg/LKT ;适宜的生根培养基为MS + 0 .1mg/LIBA ;芽诱导和快繁较适宜的条件为光照 1 6h/d、温度 ( 2 5± 1 )℃ ;在光照 1 2h/d、温度 ( 2 5± 1 )℃条件下较易生根
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| 关 键 词： | 百合 组织培养 快繁 |
| 文章编号： | 1004-3268(2004)05-0055-04 |
| 修稿时间： | 2003年11月8日 |
Studies on the Technique of Tissue Culture and Rapid Propagation of Lily |
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| WANG Yong jiang,ZHANG Zhen chen ,ZHANG Li fang,QIAO Qi,JIN Xiu lan.Studies on the Technique of Tissue Culture and Rapid Propagation of Lily[J].Journal of Henan Agricultural Sciences,2004(5):55-58. |
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| Authors: | WANG Yong jiang ZHANG Zhen chen ZHANG Li fang QIAO Qi JIN Xiu lan |
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| Institution: | WANG Yong jiang,ZHANG Zhen chen *,ZHANG Li fang,QIAO Qi,JIN Xiu lan |
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| Abstract: | Using the edible bulb of Lilum from xinyang as the explant,a method for rapid propagation of Lily was established,based on MS medium. Among 16 medium combinations with NAA,KT and 6-BA concentrations,the appropriate mediums for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L KT;The best optimum medium for differentiation and subculture was MS+1.0 mg/L 6-BA+0.01 mg/L NAA+0.5 mg/L KT.The best medium for rooting was MS+0.1 mg/L IBA.The appropriate calture condition for bud induce and rapid propagation was light period of 16 h and temp of (25±1) ℃. |
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| Keywords: | Lily Tissue culture Rapid propagation |
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