| 甘薯茎尖分生组织培养与快速繁殖技术研究※? |
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| 引用本文： | 关崇梅,秦静远,徐志英,刘玉凤,张西丽,龙金娟.甘薯茎尖分生组织培养与快速繁殖技术研究※?[J].中国农学通报,2004,20(4):33-33. |
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| 作者姓名： | 关崇梅 秦静远 徐志英 刘玉凤 张西丽 龙金娟 |
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| 作者单位： | 杨凌职业技术学院农学系,陕西,杨凌,712100 |
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| 基金项目： | 杨凌职业技术学院科研基金资助项目. |
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| 摘 要： | 甘薯茎尖分生组织培养与快速繁殖技术研究课题己进行3年,筛选出最佳培养基为MS+6-BA1.0mg/L+NAA0.1～0.2mg/L＋3%蔗糖＋琼脂6.0g/L；最适宜培养温度为26～30℃；症状学诊断法、指示植物鉴定法进行病毒检测，平均脱毒率达83.7%。脱毒试管苗切段快速增殖，繁殖系数为5n，繁殖速度以几何级数增长，一定时间内可大量繁殖脱毒苗。脱毒苗移栽大田进行产量比较，平均增产率达37.8％。
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| 关 键 词： | 甘薯 茎尖分生组织培养 快速繁殖 |
Research on Stem-apex Meristem Culture and Fast Reproduction Technology of Sweet Potato |
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| GUAN Chong-mei,QIN Jin-yuan,XU Zhi-ying,LIU Yu-feng,ZHANG Xi-li,LONG Jin-juan.Research on Stem-apex Meristem Culture and Fast Reproduction Technology of Sweet Potato[J].Chinese Agricultural Science Bulletin,2004,20(4):33-33. |
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| Authors: | GUAN Chong-mei QIN Jin-yuan XU Zhi-ying LIU Yu-feng ZHANG Xi-li LONG Jin-juan |
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| Institution: | (Yangling Vocational and Technical College, Yangling, Shaanxi 712100 China) |
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| Abstract: | The three-year research on stem-apex meristem culture and fast reproduction technology of sweet potato selected out MS+6-BA 1.0mg/L+NAA 0.1～0.2mg/L＋3%sucrose＋agar6.0g/L and 26～30℃ respectively as the preferential medium and appropriate temperature range. And the average devirus rate was 83.7% based on the semiological diagnostic and plant indicator identification. The reproduction coefficient for the fast propagation with cut test-tube plantlets was 5 n, the reproduction speed increased at a geometric progression and large-scale seedlings could be multiplied in a short time. The virus-free seedlings transplanted in fields reached a yield increase of 37.8%. |
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| Keywords: | Sweet potato stem-apex Meristem culture Rapid reproduction |
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