低温胁迫下马铃薯的数字基因表达谱分析

以马铃薯品种合作88为材料,利用数字基因表达谱(DGE)技术,对–2℃低温胁迫处理后的马铃薯叶片c DNA文库进行差异基因表达谱分析。结果表明,有28 505个基因受低温胁迫诱导差异表达,其中上调表达基因13 703个,下调表达基因14 802个。GO功能显著性富集分析表明,DEGs主要涉及信号生物代谢过程、氧化还原过程、能量代谢、次生代谢过程以及催化活性。KEGG富集分析表明,上调表达基因主要富集于苯丙烷、光合作用天线蛋白、类胡萝卜素的生物合成、苯丙氨酸代谢及淀粉与蔗糖代谢途径,而下调表达基因主要富集于植物激素信号转导途径。利用实时荧光定量PCR(q RT-PCR)验证4个DEGs在低温胁迫条件下的差异表达,其结果与DGE分析结果基本一致,证实了DGE测序结果的可靠性。

Digital Gene Expression Profiling Analysis of Potato Under Low Temperature Stress

Avariety Cooperation88was used to construct leaf cDNA library of potato treated with –2℃ low temperature stress by digital gene expression profiling (DGE) technology, and detect the differentially expressed genes(DEGs).The results of solexasequencing showed that a total of 28505 DEGs were screened out, 13703 DEGs of whichwere up-regulated and 14802 DEGs were down-regulated. GO enrichment analysis showed that these DEGs were involved insingle-organism metabolic process, oxidation-reduction process, metabolisms of carbohydrate, secondary metabolism and catalytic activity. The KEGGenrichment analysis showed that up-regulated genes were significantly associated withphenylpropanoid, photosynthesis-antennaproteins, carotenoidbiosynthesis, phenylalaninemetabolism, starch and sucrose metabolism pathways, while the down-regulated DEGs mainly enriched in plant hormone signal transduction pathway. The differential expressions of four genes were confirmed by Real-time quantitative PCR, showing that theexpression changes ofthese four genes weregenerally consistent with the results of DGE.