| 外植体和培养因子对剑麻不定芽诱导及植株再生的影响 |
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| 引用本文： | 刘巧莲,;朱军,;高建明,;张世清,;陈河龙,;郑金龙,;易克贤.外植体和培养因子对剑麻不定芽诱导及植株再生的影响[J].热带农业科学,2014(4):42-45. |
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| 作者姓名： | 刘巧莲 ;朱军 ;高建明 ;张世清 ;陈河龙 ;郑金龙 ;易克贤 |
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| 作者单位： | [1]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101; [2]中国热带农业科学院环境与植物保护研究所,海南海口571737 |
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| 基金项目： | 国家自然科学基金（No.31371679）;国家麻类产业技术体系建设专项资金（No.CARS-19-E17）;中央级公益性科研院所基本科研业务专项资金（No.ITBBI10213）;海南省自然科学基金（No.312052）. |
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| 摘 要： | 以剑麻H.11648为材料,研究不同外植体、培养基和激素对不定芽诱导及生根的影响。试验结果表明,以珠芽苗茎尖为最佳快繁材料,最佳消毒时间25 min,最适合的基本培养基为SH;最佳分化培养基为SH+NAA0.05 mg/L+6-BA 4 mg/L,芽的分化率达到85%,同时可做增殖培养基,增殖平均倍数为18。分化芽在MS和SH两种培养基中添加NAA 0.1 mg/L+6-BA 0.1 mg/L中均可生根,生根率为95%。
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| 关 键 词： | H 11648麻 快繁 MS SH |
Effects of Explants and Cultural Factors on Induction of Adventitious Shoots and Plant Regeneration of Sisal |
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| Institution: | LIU Qiaolian, Zhu Jun, GAO Jianming, ZHANG Shiqing, CHEN Helong, ZHENG Jinlong, YI Kexian (1 Institute of Tropical Bioscience and Biotechnology/Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Tropical Crops, CATAS, Haikou, Hainan 571101; 2 Institute of Environment and Plant Protection, CATAS, Haikou, Hainan 571737) |
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| Abstract: | The effects of explants and cultural factors on induction of adventitious shoots and plant regeneration of sisal H.11648 was studied. The results showed that the best explant for rapid propagation was stem tip of bead bud seedling, the suitable sterilization time was 25 min, and the most suitable basic culture medium was SH. The best medium for differentiation and proliferation was SH＋ NAA0.05 mg/L＋ 6-BA4 mg/L, bud differentiation rate reached 85%, and proliferation average was 18. Root could well developed in MS （SH）＋NAA0.1 mg/L＋6-BA0.1 mg/L and rooting rate was up to 95%. |
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| Keywords: | H 11648 hybrid sisal rapid propagation MS SH |
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