| 橡胶树树皮和木质部淀粉和可溶性糖含量测定 |
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| 引用本文： | 魏芳,;郑乾坤,;罗世巧,;仇键,;杨文凤,;吴明,;校现周.橡胶树树皮和木质部淀粉和可溶性糖含量测定[J].热带农业科学,2014(4):9-13. |
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| 作者姓名： | 魏芳 ;郑乾坤 ;罗世巧 ;仇键 ;杨文凤 ;吴明 ;校现周 |
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| 作者单位： | [1]中国热带农业科学院橡胶研究所/农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737; [2]海南大学农学院,海口570288 |
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| 基金项目： | 国家自然科学基金项目（No.31200467）：中国热带农业科学院橡胶研究所基本科研业务费专项（No.1630022012010）. |
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| 摘 要： | 提出一种橡胶树树皮和木质部非结构性碳水化合物含量测定方法。取样测定范围为10~200 mg,利用80%乙醇浸提提取可溶性糖,硫酸蒽酮法测定可溶性糖含量,硝酸钙法提取淀粉,I2-KI法测定淀粉含量。本方法优点是:取样量小,对胶树伤害小,取样过程简单,测定结果准确。
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| 关 键 词： | 橡胶树 非结构性碳水化合物 可溶性糖 淀粉 |
A Method for Measuring Soluble Sugars and Starch in Bark and Xylem of Rubber Tree |
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| Institution: | WEI Fang, ZHENG Qiankun, LUO Shiqiao, QIU Jian, YANG Wenfeng, WU Ming, XIAO Xianzhou (1 Rubber Research Institute/Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Rubber Tree, CATAS, Danzhou, Hainan 571737, China; 2 College of Agriculture, Hainan University, Haikou, Hainan 570288, China) |
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| Abstract: | A method for measurements of non-structural carbohydrates in bark and xylem of rubber tree was put forward in this study. The measuring sample was within 10-200mg, soluble sugars extracted by 80% ethanol and measured by Sulfuric acid-anthrone method, and starch extracted by Ca （NO3）2 method and measured by I2-KI. Merits of this study： little sample with little hurt to rubber tree, simple operating and accurate data. |
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| Keywords: | rubber tree non-structural carbohydrates soluble sugars starch |
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