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| 两种游离棉酚检测方法对常规棉粕检测的比较 | |
| 引用本文： | 周天兵,宋代军,李爱科.两种游离棉酚检测方法对常规棉粕检测的比较[J].畜牧与饲料科学,2011(2). |
| 作者姓名： | 周天兵 宋代军 李爱科 |
| 作者单位： | 1. 西南大学动物科技学院,重庆,400715;国家粮食局科学研究院,北京,100037 2. 西南大学动物科技学院,重庆,400715 3. 国家粮食局科学研究院,北京,100037 |
| 基金项目： | 国家十一五科技支撑计划课题(2006BAD12B04) |
| 摘 要： | 主要针对常规棉籽粕(游离棉酚含量200~1000mg/kg)中游离棉酚含量的检测,用美国油化学家学会官方方法AOCSBa7-58和国标GB13086方法作对比检测,以比较2种方法的差异和对结果的影响。参照AOCSBa7-58制定标准曲线,计算换算因子F1,AOCSBa7-58和GB13086的回收率检验,并选择3种游离棉酚含量梯度的常规棉粕测定游离棉酚含量。AOCSBa7-58标准曲线换算因子F1=0.370,R2=0.999,回收率101.14%,RSD=2.56;GB13086质量吸收系数,游离棉酚为62.5L/(cm·g),回收率104.98%,RSD=9.85。结果表明,AOCSBa7-58对棉酚及其衍生物的提取充分,检测结果稳定,重复性好;而GB13086对棉酚及其衍生物的提取不稳定,时高时低,造成检测结果的不稳定性,重复性差。建议饲料行业应由AOCSBa7-58代替GB13086检测棉粕及饲料中游离棉酚含量,更能体现棉粕及饲料中棉毒素的含量。 |
| 关 键 词： | AOCSBa7-58 GB13086 游离棉酚含量 棉粕检测 |
Detection of Free Gossypol Content in Cottonseed Meal by Two Methods |
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| ZHOU Tian-bing,SONG Dai-jun,LI Ai-ke.Detection of Free Gossypol Content in Cottonseed Meal by Two Methods[J].Animal Husbandry and Feed Science,2011(2). | |
| Authors: | ZHOU Tian-bing SONG Dai-jun LI Ai-ke |
| Institution: | ZHOU Tian-bing1,2,SONG Dai-jun1,LI Ai-ke2 (1.College of Animal Science and Technology,Southwest University,Chongqing 400715,China,2. Academy of State Administration of Grain,Beijing 100037,China) |
| Abstract: | Free gossypol content in cottonseed meals (Free gossypol content of 200 -1 000 mg/kg) was determined by AOCS recommended Ba7-58 method and national standard GB 13086 method. Referring to AOCS Ba7-58, standard curve was established, and calculate factor F1 was obtained. Rate of recovery was also determined, the free gossypol content in three cottonseed meals having different free gossypol content was determined. According to AOCS Ba7-58 standard curve, F1=0.370, R2=0.999, and rate of recovery= 101.14%. Accor... |
| Keywords: | AOCS Ba7-58 GB 13086 free gossypol content cottonseed meal detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |