| 饲用羽毛肽粉中色氨酸的测定 |
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| 引用本文： | 林香信,姚清华,宋永康.饲用羽毛肽粉中色氨酸的测定[J].畜牧与饲料科学,2011(7):39-41. |
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| 作者姓名： | 林香信 姚清华 宋永康 |
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| 作者单位： | 福建省农业科学院中心实验室,福建福州,350003 |
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| 基金项目： | 福建省属公益类项目(2009R10034-1); 福建省农业科学院青年基金(2009QB-20) |
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| 摘 要： | 比较了多种碱解剂及碱解条件对饲用羽毛肽粉中色氨酸(Trp)测定的影响。结果表明,4mol/L LiOH、(110±1)℃水解20h效果最好,其次是6mol/L NaOH+3%SnCl2、(110±1)℃水解20h。并进一步探讨了测定中碱解剂用量、碱解温度、碱解管内空气、氨的浓度等对色氨酸测定值的影响。
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| 关 键 词： | 饲用羽毛肽粉 色氨酸 氨基酸自动分析仪 碱解剂 |
Determination of Tryptophan in Forage Feather Peptide Powder |
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| LIN Xiang-xin,YAO Qing-hua,SONG Yong-kang.Determination of Tryptophan in Forage Feather Peptide Powder[J].Animal Husbandry and Feed Science,2011(7):39-41. |
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| Authors: | LIN Xiang-xin YAO Qing-hua SONG Yong-kang |
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| Institution: | LIN Xiang-xin1,2,YAO Qing-hua1,SONG Yong-kang1,2 (1.Central Laboratory,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China,2.Fujian Key Laboratory of Agricultural Testing by Precision Instruments,China) |
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| Abstract: | The effects of various alkali-hydro agents and alkali-hydrolyzable conditions on the determination of tryptophan in the forage feather peptide powder were compared. The results showed that the effect of hydrolyzing with 4 mol/L LiOH at（110±1） ℃ for 20 h was the best,the effect of hydrolyzing with 6 mol/L NaOH and 3% SnCl2 at（110±1） ℃ for 20 h was the next. The effects of the dose of alkali-hydro agent,alkali-hydrolyzable temperature,the concentration of atmosphere and ammonia in the alkali-hydrolyzable tube on the determined value of tryptophan in the determination were further discussed. |
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| Keywords: | forage feather peptide powder tryptophan automatic amino acid analyzer alkali-hydro agent |
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