| 海兰褐鸡法氏囊中Cu/Zn-SOD、Mn-SOD及NOS cDNA克隆及序列分析 |
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| 引用本文： | 景龙,李立辉,刘畅,张健,邵悦,滕素玲,郑艳生,郑世民.海兰褐鸡法氏囊中Cu/Zn-SOD、Mn-SOD及NOS cDNA克隆及序列分析[J].中国家禽,2011,33(17). |
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| 作者姓名： | 景龙 李立辉 刘畅 张健 邵悦 滕素玲 郑艳生 郑世民 |
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| 作者单位： | 东北农业大学动物医学学院动物病理生理教研室,黑龙江哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金(30972162) |
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| 摘 要： | 通过对海兰褐鸡法氏囊中Cu/Zn-SOD、Mn-SOD及iNOS cDNA克隆及序列分析,了解海兰褐鸡法氏囊中主要氧化-抗氧化酶的分布及其基因组成,为研究传染性法氏囊病及与法氏囊相关禽病的氧化-抗氧化发病机制奠定基础.结果显示,Cu/Zn-SOD、Mn-SODcDNA序列分别出现2个碱基的突变,iNOS cDNA序列与原始序列同源性为100％.海兰褐鸡法氏囊中存在Cu/Zn-SOD、Mn-SOD及iNOS 3种与氧化-抗氧化相关酶,并且Cu/Zn-SOD、Mn-SOD cDNA序列具有新特征,iNOS在海兰褐鸡体内是稳定存在的；海兰褐鸡可能与康奈尔K系鸡具有相似的遗传背景.
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| 关 键 词： | 海兰褐鸡 法氏囊 Cu/Zn-SOD Mn-SOD iNOS |
Cloning and Sequence Analysis of Cu/Zn-SOD,Mn-SOD and iNOS cDNA in Bursal of Hy-line Brown Roosters |
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| JING Long,LI Lihui,LIU Chang,ZHANG Jian,SHAO Yue,TENG Suling,ZHENG Yansheng,ZHENG Shimin.Cloning and Sequence Analysis of Cu/Zn-SOD,Mn-SOD and iNOS cDNA in Bursal of Hy-line Brown Roosters[J].China Poultry,2011,33(17). |
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| Authors: | JING Long LI Lihui LIU Chang ZHANG Jian SHAO Yue TENG Suling ZHENG Yansheng ZHENG Shimin |
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| Institution: | JING Long,LI Lihui,LIU Chang,ZHANG Jian,SHAO Yue,TENG Suling,ZHENG Yansheng,ZHENG Shimin**(Department of Pathophysiology,College of Veterinary Medicine,Northeast Agricultural University,Harbin,Heilongjiang 150030) |
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| Abstract: | By cloning and sequence analysis of copper/zine superoxide dismutase(Cu/Zn-SOD),manganese superoxide dismutase(Mn-SOD) and inducible nitric synthase(iNOS) cDNA in bursal of Hy-line brown roosters,distribution and constitution of gene of the oxidant/antioxidant enzymes in bursal were studied.It was the basis for oxidant/antioxidant mechanism of infectious bursal disease and bursal-related disease.The results showed that there were two base changed in Cu/Zn-SOD cDNA and Mn-SOD cDNA,but the sequence of iNOS cD... |
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| Keywords: | Hy-line brown roosters bursal Cu/Zn-SOD Mn-SOD iNOS |
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