| 一株土壤中苯酚降解菌的分离、鉴定及降解特性研究 |
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| 引用本文： | 崔树军,金维平,张建云,陈红歌,张庆甫. 一株土壤中苯酚降解菌的分离、鉴定及降解特性研究[J]. 河南农业科学, 2009, 0(4) |
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| 作者姓名： | 崔树军 金维平 张建云 陈红歌 张庆甫 |
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| 作者单位： | 1. 河南工程学院,资源与环境工程系,河南,郑州,451191
2. 河南农业大学,生命科学学院,河南,郑州,450002 |
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| 基金项目： | 河南工程学院青年基金 |
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| 摘 要： | 采用富集培养的方法,从焦化废水污染的土壤中分离得到1株能够高效降解苯酚的菌株,命名为HNGCXY.1。该菌株可以在以苯酚为惟一碳源和能源的无机培养基上生长,能够耐受最高浓度为1000 mg/L的苯酚。对该菌株的降解性能研究表明:该菌株具有较强的苯酚降解能力,在温度为28~32℃,pH值为6.5~7.5,摇床振荡速度大于160 r/min,苯酚浓度为600 mg/L的条件下,单位时间内对苯酚的降解能力最强。根据其生理生化特性和16S rDNA序列同源性分析结果,将其初步鉴定为产碱杆菌(Alcaligenessp.)。
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| 关 键 词： | 苯酚降解菌 产碱杆菌 分离鉴定 16SrDNA |
Studies on Isolation and Degradation Characteristics of A Phenol Degrading Bacteria |
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| CUI Shu-jun,JIN Wei-ping,ZHANG Jian-yun,CHEN Hong-ge,ZHANG Qing-fu. Studies on Isolation and Degradation Characteristics of A Phenol Degrading Bacteria[J]. Journal of Henan Agricultural Sciences, 2009, 0(4) |
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| Authors: | CUI Shu-jun JIN Wei-ping ZHANG Jian-yun CHEN Hong-ge ZHANG Qing-fu |
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| Affiliation: | 1.College of Resource and Environment Science;Henan Institute of Engineering;Zhengzhou 451191;China;2.College of Life Science;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | A strain(HNGCXY.1) isolated from the phenol-contaminated soil of a coking plant was able to grow on phenol as sole carbon and energy sources.The highest endurance capability of this strain for phenol was 1000mg/L.The factors affecting phenol degrading were investigated.It showed better phenol degrading rate at pH 6.5-7.5,28-32℃,rotation speed bigger than 160r/min,the concentration of 600mg/L phenol as the substrate.It was identified as Alcaligenes sp.,based on the 16s rDNA sequences,biochemical and physiological characteristics. |
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| Keywords: | 16S rDNA |
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