基于混合培养和PCR-变性梯度凝胶电泳的复合净水功能菌群构建

以11株具有不同净水功能的菌株为出发菌株,采用液体共培养的方法,应用PCR-变性梯度凝胶电泳(DGGE)分析混合培养物中的菌群变化,确定这些菌株的共存能力和生长特性。以混合培养中的优势菌株为核心形成不同菌群组合,确定复配菌株组合,并通过净水实验确定其净水功能。结果显示,菌株AOZ1或BSK9、W14或BSK3在混合培养条件下可以有效生长,是混合培养的优势功能菌。以菌株AOZ1或BSK9为核心菌株,与其他功能菌组合可构建形成复合净水功能菌。其中,由AOZ1、BSK9、W14和BSK3组成的复合菌对河道污水中的化学需氧量(COD)和氨氮具有较高的去除率,分别达到48.0%和76.8%。结果表明,采用PCR-DGGE监测下的液体共培养方法,可有效构建净水复合菌群。

Construction of compound bacteria for polluted water purification based on mixed culture and PCR-denaturing gradient gel electrophoresis

In the current study, 11 strains with different water purification functions were chose to be cultured together in LB medium, and PCR-denaturing gradient gel electrophoresis (DGGE) was used to reveal the changes of bacterial community after co-culturing to determine the coexistence and growth characteristics of these strains. The dominant strains in mixed culture were used as the core to form different compound bacteria, and their functional effects were determined through water purification experiments. The results showed that strains AOZ1 or BSK9, W14 or BSK3 could grow effectively under mixed culture conditions, and were the dominant bacteria in mixed culture. With strains AOZ1 or BSK9 as the core strains, the compound water purification functional bacteria could be constructed. Among them, the compound bacteria composed of strain AOZ1, BSK9, W14 and BSK3 had high removal rates of chemical oxygen demand (COD) and ammonia nitrogen, which reached 48.0% and 76.8%, respectively. The results suggested that compound water purification bacteria could be effectively constructed by the liquid co-culture method along with PCR-DGGE.