| 发芽糙米γ-氨基丁酸的检测及研究进展 |
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| 引用本文： | 刘红梅,魏淘涛,刘行丹,刘建丰.发芽糙米γ-氨基丁酸的检测及研究进展[J].作物研究,2012(1):88-92. |
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| 作者姓名： | 刘红梅 魏淘涛 刘行丹 刘建丰 |
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| 作者单位： | 湖南农业大学农学院／作物生理与分子生物学教育部重点实验室,湖南长沙410128 |
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| 基金项目： | 国家863计划项目（2010AAl01304）;湖南省大学生创新性实验项目（SCXlll0）. |
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| 摘 要： | 介绍了发芽糙米γ-氨基丁酸（GABA）含量的各种检测方法，重点介绍了Berthelot改良比色法、改良纸层析法、氨基酸分析仪和柱前衍生液相色谱等4种常用的方法。综述了国内外对发芽糙米GABA积累机理．发芽条件和工艺参数的研究情况。提出了根据试验需要选择合理的GABA检测方法的建议。应对不同水稻品种发芽糙米中GABA含量差异形成的原因及基因型进行遗传分析，以筛选出高GABA含量和高谷氨酸脱羧酶活性的品种，为农产品深加工提供优质原材料。
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| 关 键 词： | 发芽糙米 γ-氨基丁酸 检测方法 |
Determination Methods and Research Progress of Gamma- Aminobutyric Acid in Germinated Brown Rice |
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| LIU Hong-mei,WEI Tao-tao,LIU Xing-dan,LIU Jian-feng.Determination Methods and Research Progress of Gamma- Aminobutyric Acid in Germinated Brown Rice[J].Crop Research,2012(1):88-92. |
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| Authors: | LIU Hong-mei WEI Tao-tao LIU Xing-dan LIU Jian-feng |
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| Institution: | LIU Hong - mei, WEI Tao - tao, LIU Xing - dan, LIU Jian - feng ( Key Laboratory of Crop Physiology and Molecular Biology, Ministry of Education/ College of Aga＇onomy, Hunan Agricultural University, Changsha, Hunan 410128, China) |
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| Abstract: | Four methods of gamma- aminobutyric acid （GABA） determination including Berthelot modified colorimetric method, modified paper chromatography, amino acid analyzer method and derivative high performance liquid chromatogra- phy were reviewed and evaluated in this paper. Studies on GABA accumulation mechanism in germinated brown rice, germination conditions and process parameters at home and abroad were summarized. Different determination methods should be elected according to experimental requirement. Different rice varieties or different genetic types should be analyzed in order to screen rice varieties with high GABA content and high glutamate decarboxylation enzyme activity to supply high quality raw materials for deep processing of agricultural products. |
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| Keywords: | Germinated brown rice GABA Determination method |
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