Effect of pre-incubation humidity and temperature treatment on the in vitro germination of avocado pollen grains |
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Authors: | Marianthi Loupassaki Miltiadis Vasilakakis Ioannis Androulakis |
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Institution: | (1) Subtropical Plants and Olive Trees Institute, 73100 Chania, Crete, Greece;(2) Div. of Plant Science, Aristotelian University of Thessaloniki, 54006 Thessaloniki, Greece |
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Abstract: | Optimum in vitro germination of pollen grain of the avocado cultivars Fuerte, Nabal, Ettinger, Bacon and Zutano occurred at
25 °C. However, there were significant differences between cultivars in percentage germination and relative humidity (RH)
requirements for optimum pollen grain growth. The most sensitive cultivar to relative humidity was Fuerte, in which the germination
of pollen grain rose from 11.4%, at 40% RH, to about 50%, after one hour at 100% RH. The germination% of Nabal pollen grain
was already high at 40% RH and was not increased by higher relative humidity. Increased relative humidity also helped to sustain
the viability of avocado pollen. At 30 °C and 5% RH the pollen grains of Fuerte quickly lost its ability to germinate, at
40% RH for 1 hour, germination was reduced spectacularly compared to pollen kept in saturated with moisture environment where
it was not affected the first 24 hours. The effects of temperature and relative humidity on fruit-set and yield of avocado
are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Persea americana avocado humidity Greece pollen grain germination temperature fruit-set |
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