The quality after culture in vitro or in vivo of porcine oocytes matured and fertilized in vitro and their ability to develop to term |
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Authors: | Yoshiyuki Nakamura Sigeyuki Tajima Kazuhiro Kikuchi |
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Institution: | 1. Saitama Prefectural Agricultural Technology Research Center, Kumagaya, Japan;2. Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan;3. The United Graduate School of Veterinary Science, Yamaguchi University, Yamguchi, Japan;4. Aichi Prefectural Agriculture and Forestry Research Center, Nagakute, Japan |
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Abstract: | The quality of porcine blastocysts produced in vitro is poor in comparison with those that develop in vivo. We examined the quality of in vitro‐matured and fertilized (IVM/IVF) oocytes, their abilities to develop to blastocysts under in vivo and in vitro conditions, and the potential of the embryos to develop to term after transfer. IVM/IVF oocytes were either transferred and the embryos recovered on Days 5 and 6 (100% and 87.5%, respectively) (‘ET‐vivo’ embryos), or cultured in vitro for 5 or 6 days (‘IVC’ embryos). The proportion of blastocysts differed significantly between the two groups on Day 5 (20.6% and 8.0%, respectively), but not on Day 6 (23.8% and 21.2%, respectively). The mean number of cells in ET‐vivo blastocysts on Days 5 or 6 was significantly higher (72.8 and 78.7, respectively) than that in IVC blastocysts (22.1 and 39.7, respectively). When IVM/IVF oocytes and IVC blastocysts on Day 6 were transferred, all (three and three, respectively) developed to piglets (16 and 16, respectively), without any difference in the rates of development to term (2.1% and 2.6%, respectively). These data suggest that, although blastocyst production differs between the two culture conditions, IVM/IVF oocytes possess the same ability to develop to term. |
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Keywords: | blastocyst culture embryo transfer
in  vitro
pig |
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