不同基质中香菇菌丝生长期胞外酶活性变化的研究

目的]通过分析不同基质中香菇菌丝生长期4种胞外酶的活性变化情况,为香菇营养生理研究提供参考.方法]提取不同基质中的粗酶液,利用分光光度计测定光密度值,分析羧甲基纤维素酶、滤纸纤维素酶、淀粉酶及漆酶的活性变化.结果]不同基质对胞外酶活性大小有影响;各种酶活性高峰期不同,羧甲基纤维素酶和滤纸纤维素酶的活性高峰出现在转色末期;淀粉酶的活性高峰位于转色初期;漆酶的活性高峰在发菌初期.结论]胞外酶在不同基质和菌丝不同生长阶段活性存在差异,但同一种酶在不同基质中的活性变化趋势总体一致.

Study on Changes of Extracellular Enzyme Activities during Different Growth Stages of Lentinus edodes Mycelium in Different Culture Substrates

Objective] The changes of extracellular enzyme activity during different growth stages of Lentinus edodes mycelium in different culture substrates were analyzed to provide the references for study on nutritional physiology of Lentinus edodes.Method] Enzyme liquid was extracted from different culture substrates.The optical density value was determined using spectrophotometer.The changes in activity of carboxymethyl cellulase,cellulase,amylase,and laccase were analyzed.Result] Different culture substrates had different effects on the activity of extracellular enzyme.The peak activities of various enzymes varied.The peak activity of carboxymethyl cellulase enzyme and cellulase filter paper enzyme occured at the end of color changing period.The peak activity of amylase occured at the beginning.The peak activity of laccase occured at the beginning of germinating.Conclusion]There were differences of extracellular enzyme activity in different culture substrates and different growth stages.The trend of activity changes of each enzyme was similar in different culture substrates.