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家鹑与野生日本鸣鹑群体微卫星DNA标记的遗传学分析
引用本文:王惠影,常洪,徐伟,常国斌.家鹑与野生日本鸣鹑群体微卫星DNA标记的遗传学分析[J].畜牧兽医学报,2004,35(4):367-371.
作者姓名:王惠影  常洪  徐伟  常国斌
作者单位:扬州大学动物科学与技术学院,扬州,225009
基金项目:国家自然科学基金资助项目(30070550)
摘    要:选择10个微卫星标记位点,合成引物对微山湖野生日本鸣鹑和家鹌鹑各40只进行PCR扩增,变性聚丙烯酰胺凝胶电泳检测分型,并对其品种内和品种间的遗传变异进行群体遗传学分析,从分子水平上揭示这两个鹌鹁群体的遗传结构关系、亲缘关系和遗传分化程度,为鹌鹑的遗传资源研究提供新资料。

关 键 词:家鹑  野生日本鸣鹑  群体  微卫星DNA标记  遗传学
文章编号:0366-6964(2004)04-0367-05

Genetic Analysis of Microsatellite DNA Markers in Domestic Quail and Wild Japanese Quail Populations
WANG Hui-ying,CHANG Hong,XU Wei,CHANG Guo-bin.Genetic Analysis of Microsatellite DNA Markers in Domestic Quail and Wild Japanese Quail Populations[J].Acta Veterinaria et Zootechnica Sinica,2004,35(4):367-371.
Authors:WANG Hui-ying  CHANG Hong  XU Wei  CHANG Guo-bin
Abstract:In this study ten primer pairs were tested by polymerase chain reaction (PCR) and denaturing gel electrophoresis for domestic quail and wild Japanese quail microsatellite sites, forty for each type.Meanwhile, the genetic diversity within and between population was also analyzed. All the results manifested the genetic structure, phylogenetic relationship and genetic divergency between the two quail populations at molecular biotechnique level. Furthermore, it provided new data for the research of quail resource.
Keywords:microsatellite DNA markers  domestic quail  wild Japanese quail  Weishan Lake area  genetic divergency
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