| 地高辛标记cDNA探针检测苹果茎痘病毒 |
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| 引用本文： | 杨俊玲,董雅凤,李世访,张尊平,何峻.地高辛标记cDNA探针检测苹果茎痘病毒[J].植物病理学报,2006,36(5):470-472. |
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| 作者姓名： | 杨俊玲 董雅凤 李世访 张尊平 何峻 |
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| 作者单位： | 1 中国农业科学院果树研究所, 辽宁兴城 125100;2 中国农业科学院植物保护研究所, 北京 100094 |
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| 基金项目： | 国家重点实验室基金;中国农业科学院科研项目 |
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| 摘 要： | Partial sequence(314 bp) of ASPV was cloned and used as a probe labelled with digoxigenin-11dUTP. The total RNA extracted from samples with Apple stem pitting virus and a series of dilutions of plasmid with ASPV-cDNA were detected by dot blot hybridization. The results showed that the probe was sensitive and specific. The probe couldn't hybridize with total RNA of Apple stem grooving virus, Apple mosaic virus and Apple chlorotic leaf spot virus samples as well as negative control, only hybridized with that extracted from dormant shoot infected with ASPV. The sensitivity for detection of plasmid contained ASPV-cDNA was 1.64 μg.
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| 关 键 词： | 苹果茎痘病毒 cDNA探针 地高辛标记 检测 无病毒苗木 病毒病害 virus stem |
| 文章编号： | 0412-0914(2006)05-0470-03 |
| 修稿时间： | 2005年8月11日 |
Detection of Apple stem pitting virus (ASPV) by dot blot hybridization with digoxigenin-labelled viral cDNA probe |
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| YANG Jun-ling,DONG Ya-feng,LI Shi-fang,ZHANG Zun-ping,HE Jun.Detection of Apple stem pitting virus (ASPV) by dot blot hybridization with digoxigenin-labelled viral cDNA probe[J].Acta Phytopathologica Sinica,2006,36(5):470-472. |
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| Authors: | YANG Jun-ling DONG Ya-feng LI Shi-fang ZHANG Zun-ping HE Jun |
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| Institution: | 1 Research Institute of Pomology, Chinese Academy of Agricultural Science, Liaoning, Xingcheng 125100, China;2 Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100094, China |
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| Abstract: | Partial sequence(314 bp) of ASPV was cloned and used as a probe labelled with digoxigenin-11dUTP. The total RNA extracted from samples with Apple stem pitting virus and a series of dilutions of plasmid with ASPV-cDNA were detected by dot blot hybridization. The results showed that the probe was sensitive and specific. The probe couldn't hybridize with total RNA of Apple stem grooving virus, Apple mosaic virus and Apple chlorotic leaf spot virus samples as well as negative control, only hybridized with that extracted from dormant shoot infected with ASPV. The sensitivity for detection of plasmid contained ASPV-cDNA was 1.64 μg. |
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| Keywords: | Apple stem pitting virus digoxigenin-labelled probe dot blot hybridization detection |
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