| 1株牛源蜡样芽孢杆菌的分离鉴定及毒力基因检测 |
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| 引用本文： | 宋丽丽,张玲艳,贾伟娟,白志恒,刘 媛,陈云娇,王学理. 1株牛源蜡样芽孢杆菌的分离鉴定及毒力基因检测[J]. 西北农业学报, 2019, 28(11): 1735-1741 |
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| 作者姓名： | 宋丽丽 张玲艳 贾伟娟 白志恒 刘 媛 陈云娇 王学理 |
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| 作者单位： | （内蒙古民族大学 动物科学技术学院，内蒙古通辽 028042） |
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| 基金项目： | 内蒙古自治区高等学校科学技术研究重点项目（NJZZ19147）;内蒙古自治区肉牛疾病防控工程技术研究中心开放课题（MDK2019022）。 |
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| 摘 要： | 从一头猝死牛的脾脏内分离得到1株细菌,对该菌染色镜检可见其为革兰氏阳性杆菌,呈单个或链状排列。该菌对头孢氨苄、庆大霉素、诺氟沙星等药物敏感。生化检测结果和16S rDNA序列分析表明,该菌株为蜡样芽孢杆菌;经PCR检测该菌含有hbl、nhe、entFM等毒素基因以及看家基因groEL。动物致病性试验结果显示,该菌具有较强的致病性。上述结果表明,该分离菌为致病性蜡样芽孢杆菌。
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| 关 键 词： | 牛；蜡样芽孢杆菌；分离鉴定；毒力基因 |
Isolation, Identification and Virulence Gene Detection of Bacillus cereusin Cattle |
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| SONG Lili,ZHANG Lingyan,JIA Weijuan,BAI Zhiheng,LIU Yuan,CHEN Yunjiao and WANG Xueli. Isolation, Identification and Virulence Gene Detection of Bacillus cereusin Cattle[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2019, 28(11): 1735-1741 |
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| Authors: | SONG Lili ZHANG Lingyan JIA Weijuan BAI Zhiheng LIU Yuan CHEN Yunjiao WANG Xueli |
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| Abstract: | A strain of bacteria was isolated from the spleen of a sudden death bovine, which was Gram-positive bacilli with single or chain arrangement, and was sensitive to Cefalexin, gentamicin, norfloxacin and so on. The results of biochemical test and 16S rDNA sequence analysis showed that the strain was Bacillus cereus, and the strain contained toxin genes such as hbl, nhe, entFM, housekeeping gene groEL.The results of animal pathogenicity test showed that the strain had strong pathogenicity. The all results showed that the isolated strain was pathogenic Bacillus cereus. |
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| Keywords: | Bovine Bacillus cereus Isolation and identification Virulence gene |
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