月季切花乙烯受体ETR1 cDNA克隆及其序列分析*

 根据乙烯受体基因ETR1 保守区设计引物, 分别以瓶插寿命差异显著的月季切花品种‘德克萨斯’和‘维亚蒂’为材料, 通过RT-PCR 从花瓣中扩增出了797 bp 的cDNA 片段, 它编码265 个氨基酸。测序和序列分析表明,‘德克萨斯’重组质粒中插入片段的核苷酸序列之间完全相同, 命名为pRT-ETR1。而‘维亚蒂’所获得的重组质粒中插入片段的核苷酸和氨基酸序列之间存在差异, 同源性分别为85. 2 %和92. 1 % , 分别命名为pRV-ETR1-V4 和pRV-ETR1-V5。pRV2 ETR12V5 的核苷酸和氨基酸序列与‘德克萨斯’pRT2 ETR1 的同源性均达99 %以上; pRV-ETR1-V4 中的核苷酸和氨基酸序列与‘德克萨斯’pRT-ETR1 的同源性分别为85. 0 %和92. 5 %。上述插入片段与桃、苹果、天竺葵、拟南芥等植物的ETR1相应区域高度同源, 其氨基酸同源性均大于90 %。

Cloning and Sequence Analysis of Ethylene Receptor ETR1 cDNA from Cut Roses

The primers were designed according to the conservative domain of ETR1 cDNA from other heterogeneous plants.The fragments of 797bp cDNA,which encoding 265 amino acid residues were amplified through RT-PCR from petals of cut roses'Texas'and'Viraldi'with different vase lives.Sequence analyses show that the nucleotides of insert fragments in recombinants of'Texas'are completely identical;which named pRT-ETR1.Sequence homologies of nucleotide and deduced amino acid residues between two kinds of recombination of'Viraldi'are 85.2% and 92.5% respectively,the two kinds of recombination were named pRV-ETR1-V4 and pRV-ETR1-V5.The nucleotide sequence of pRV-ETR1-V5 is 99% of homology with that of'Texas'.Sequence homologies of nucleotide and deduced amino acid residues between pRV-ETR1-V4 and those of'Texas'are 85.0% and 92.5% respectively.Compared with other heterogeneous high plants,all of the above fragments of ETR1 cDNA of cut roses are highly homologous with other high plants,such as peach,apple, Arabidopsis and geranium,and homology of amino residues are all beyond 90%.