猴丰1号新菌株的选育及其酯酶同工酶分析

采用子实体组织分离与系统筛选法,从福建猴头的变异株中选育出了定名为“猴丰1号”的新菌株。该菌株菌丝体生长势强,发菌迅速;子实休菇形大,产量高。鲜菇产量比出发株福建猴头增加69.3％,比著名的常山99菌株增加16.8％。酯酶同工酶分析显示:猴丰1号的酯酶谱型与福建猴头相似,但有1条酶带发生了变异。由此说明,它确是源于福建猴头的自然变异株。

The Selection of Houfeng No. 1 New Strain and Analysis of Its Esterase Isozyme

Houfeng No. 1 strain was selected from the mutant of Fujian Hericium erinaceus by the methods of tissue isolation and systematic screening. Its mycelium has powerful growth vigour with high spawn run rate. The fruitbody is big and high-yielding. Comparison experiments of 12 strains showed that Houfeng No. 1 was the first in yield of fresh fruitbody, dry matter rate and biological efficiency. Its fresh fruitbody yield was 69.3% and 16.8% higher than that of Fujian H. erinaceus and Changshan 99 respectively. Analysis of esterase isozyme exhibited that both Houfeng No. 1 and Fujian H. erinaceus had 4 enzyme bands. Three of them were the same in width, colour and Rf value; however, one of them was different not only in the Rf value, but also in the width and the colour. Thus it was proved that Houfeng No. 1 was a truly natural mutant from Fujian H. erinaceus.