| 蒺藜苜蓿二氢黄酮还原酶基因克隆及植物表达载体构建 |
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| 引用本文： | 王延秀,张金文,武禄光.蒺藜苜蓿二氢黄酮还原酶基因克隆及植物表达载体构建[J].甘肃农业大学学报,2009,44(5). |
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| 作者姓名： | 王延秀 张金文 武禄光 |
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| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070
2. 昆士兰大学生命学院,澳大利亚,昆士兰,4072 |
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| 摘 要： | 采用RT-PCR方法,从蒺藜苜蓿(Medicargotrunctula)中克隆了二氢黄酮还原酶(DFR)基因cDNA片段,并进行DFR基因植物表达载体的构建.结果表明:扩增的DFR基因片段全长约1 000 bp,编码337个氨基酸,与Gen-Bank中已注册的DFR基因核苷酸序列的同源性为99.80%.以pBI121为基础载体,构建了CaMV35S启动子驱动的DFR基因的植物表达载体pBIDFR,然后导入农杆菌EHA105,经PCR验证确定构建出植物表达载体.
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| 关 键 词： | 蒺藜苜蓿 二氢黄酮还原酶 基因克隆 表达载体 |
Cloning and construction of plant expression vector of dihydroflavonol reductase gene from Medicargo truncatula |
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| WANG Yan-xiu,ZHANG Jin-Wen,WU Lu-guang.Cloning and construction of plant expression vector of dihydroflavonol reductase gene from Medicargo truncatula[J].Journal of Gansu Agricultural University,2009,44(5). |
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| Authors: | WANG Yan-xiu ZHANG Jin-Wen WU Lu-guang |
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| Institution: | WANG Yan-xiu1,ZHANG Jin-Wen1,WU Lu-guang2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China,2 College of Life,Queensland University,Queensland 4072,Australia) |
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| Abstract: | In this study,RT-PCR was employed to clone cDNA fragment of dihydroflavonol reductase(DFR) gene of 1 000 bp from Medicargo truncatula;Sequence analysis showed that 99.8 % of the DFR were homologous to those in GenBank.And this sequence encoded 337 amino-acid residues.Plant expression vector named pBIDFR was constructed based on pBI121 vector.Moreover,pBIDFR was transferred into Agrobacterium tumefacien EHA105 by direct DNA transfer. |
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| Keywords: | Medicargo trunctula dihydroflavonol reductase gene cloning expression vector |
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