| 白来航鸡IL-18和GM-CSF基因克隆与序列分析 |
| |
| 引用本文： | 于新友,赵蕾,孙翠萍,沈志强.白来航鸡IL-18和GM-CSF基因克隆与序列分析[J].中国家禽,2011,33(11). |
| |
| 作者姓名： | 于新友 赵蕾 孙翠萍 沈志强 |
| |
| 作者单位： | 1. 山东绿都生物科技有限公司,山东滨州,256600
2. 山东绿都生物科技有限公司,山东滨州256600;山东省滨州畜牧兽医研究院,山东滨州256600 |
| |
| 摘 要： | 根据GenBank中鸡白细胞介素18(IL-18)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)基因的序列设计2对特异引物,以白来航鸡脾淋巴细胞总RNA为模板进行RT-PCR扩增,克隆白来航鸡IL-18和GM-CSF工基因并进行序列分析.测序结果表明,白来航鸡IL-18基因开放阅读框为597 bp,编码199个氨基酸;序列分析表明,所获得的白来航鸡IL-18基因与GenBank中鸡IL-18的核苷酸同源性为99.0%～99.8%,氨基酸同源性为97.5%～99.5%.白来航鸡GM-CSF基因开放阅读框为435 bp,编码145个氨基酸;序列分析表明,所获得的白来航鸡GM-CSF基因与GenBank鸡GM-CSF的核苷酸同源性为99.3%～100%,氨基酸同源性为99.3%～100%,生物信息学分析表明,IL-18与GM-CSF基因编码的蛋白具有亲水性,都有很强的抗原性,IL-18共有2个潜在的N-糖基化位点,可能存在4个蛋白激酶C磷酸化位点,存在3个酪蛋白激酶Ⅱ磷酸化位点;GM-CSF共有1个潜在的N-糖基化位点,可能存在1个蛋白激酶C磷酸化位点,存在4个酪蛋白激酶Ⅱ磷酸化位点.
|
| 关 键 词： | 白细胞介素-18 粒细胞-巨噬细胞集落刺激因子 序列分析 |
Cloning and Sequence Analysis of IL-18 and GM-CSF Gene in White Leghorn Chickens |
| |
| YU Xinyou,ZHAO Lei,SUN Cuiping,SHEN Zhiqiang.Cloning and Sequence Analysis of IL-18 and GM-CSF Gene in White Leghorn Chickens[J].China Poultry,2011,33(11). |
| |
| Authors: | YU Xinyou ZHAO Lei SUN Cuiping SHEN Zhiqiang |
| |
| Institution: | YU Xinyou1,ZHAO Lei1,2,SUN Cuiping1,SHEN Zhiqiang1,2(1.Shandong Lvdu Bio-Industry Co.,Ltd.,Binzhou,Shandong 256600,2.Shandong Binzhou Animal Science and Veterinary Medicine Academy,Shandong 256600) |
| |
| Abstract: | Two pair of primers were designed according to interleukin-18(IL-18)and granulocyte-macrophage colony stimulating factor(GM-CSF)gene sequences of chicken registered in GenBank.Total RNA from spleen lymphocyte in white Leghorn was taken as template for RT-PCR amplification.IL-18 and GM-CSF genes in white Leghorn were cloned and sequenced.The sequencing results showed that the open reading frame of IL-18 gene in white Leghorn was 597 bp,coding 199 amino acids.The sequence analysis showed the nucleotide homolo... |
| |
| Keywords: | IL-18 GM-CSF sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
