Anti-diabetic effects of emodin involved in the activation of PPARγ on high-fat diet-fed and low dose of streptozotocin-induced diabetic mice |
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Authors: | Jianfeng Xue Wenjun Ding Yan Liu |
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Institution: | 1. College of Life Sciences, Graduate University of Chinese Academy of Sciences, No 19 A, Yu Quan Road, Beijing 100049, China;2. Xinjiang Institute of Ecology and Geography of Chinese Academy of Sciences, Urumqi, Xinjiang 830011, China |
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Abstract: | Rheum palmatum Linn has been widely applied in the clinical treatment of diabetes mellitus. It has been found that emodin as the major bioactive component of R. palmatum L exhibits the competency to activate peroxisomal proliferator-activated receptor-γ (PPARγ) in vitro. So the aim of this study was to evaluate the anti-diabetic effects of emodin through the activation of PPARγ on high-fat diet-fed and low dose of streptozotocin (STZ)-induced diabetic mice. The diabetic mice were intraperitoneally injected with emodin for three weeks. No changes of food consumption and the body weight in emodin-treated mice were monitored daily during the entire experiment. At the end of experiment, the levels of blood glucose, triglyceride and total cholesterol in serum were significantly decreased after emodin treatment. However, serum high-density lipoprotein cholesterol (HDLc) concentration was significantly elevated. The glucose tolerance and insulin sensitivity in emodin-treated group were significantly improved. Furthermore, the results of quantitative RT-PCR analysis showed that emodin significantly elevated the mRNA expression level of PPARγ and regulated the mRNA expressions of LPL, FAT/CD36, resistin and FABPs (ap2) in liver and adipocyte tissues. No effects on the mRNA expressions of PPARα and PPARα-target genes were observed. Taken together, the results suggested that the activation of PPARγ and the modulation of metabolism-related genes were likely involved in the anti-diabetic effects of emodin. |
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Keywords: | ALT Alanine aminotransferase FABPs (ap2) Adipocyte fatty acid binding protein FAT/CD36 Fatty acid translocase GTT Glucose tolerance test HDLc High-density lipoprotein cholesterol ITT Insulin tolerance test LDLc Low-density lipoprotein cholesterol LPL Lipoprotein lipase LSD Least significant difference PPARs Peroxisome proliferator-activated receptors PPARγ Peroxisome proliferator-activated receptor-γ STZ Streptozotocin TZDs Thiazolidinediones UCP Uncoupling protein |
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